Characterization of a Mutant of Thiostrepton-Producing Streptomyces azureus ATCC 14921 and Its Plasmids
スポンサーリンク
概要
- 論文の詳細を見る
A mutant, strain PK10,of Streptomyces azureus ATCC 14921 and its two plasmids were characterized and compared with another mutant, PK100,and its plasmid. One PK10 plasmid of 8.8 kb was identical to a pock-forming plasmid, pSA1.1,of PK100. The other plasmid which was found only in PK10 and named pSA1.2 (size, 7.6 kb), was a non-pock forming derivative of pSA1.1 with deletions in two different regions (about 1.2 kb and 30 b long). The pock-forming ability of strain PK10 on a plasmid-free strain was lower than that of strain PK100 which contained only pSA1.1. Strain PK10 had femer copies of pSA1.1 than strain PK100,and had normal spore formation and thiostrepton production, which were depressed in the strain PK100. The pSA1.1 from both PK10 and PK100 amplified to 20 to 30 copies in the transformants and inhibited their spore formation and thiostrepton production. Thus, the function of pSA1.1 appeared to be depressed by pSA1.2.
- 社団法人日本生物工学会の論文
- 1988-04-25
著者
-
Hayashida Shinsaku
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kyushu University
-
Ogata Seiya
Microbial Genetics Division, Institute of Genetic Resources,Kyushu University
-
Ogata Seiya
Microbial Genetics Division Institute Of Genetic Resources Kyushu University
-
Kinoshita Chizuru
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kyushu University
-
Ogata Seiya
Microbial Genetic Division Institute Of Genetic Resources
-
MIYOSHI YUKO
Laboratory of Applied Microbiology, Department of Agricultural Chemistry Kyushu University
-
Miyoshi Yuko
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kyushu University
関連論文
- Linear and Circular Conformation of Pock-Forming Plasmid pSAl of Thiostrepton-Producing Streptomyces azureus ATCC 14921
- Isolation of Anti-Algal Pseudomonas stutzeri Strains and Their Lethal Activity for Chattonella antiqua(Microbiology & Fermentation Industry)
- Role of the Carbohydrate Moiety of a Glucoamylase from Aspergillus awamori var.kawachi in the Digestion of Raw Starch
- The Mechanism of Binding of Glucoamylase I from Aspergillus awamori var. kawachi to Cyclodextrins and Raw Starch
- Construction of Shuttle Vector Plasmid Between Clostridium acetobutylicum and Escherichia coli(Microbiology & Fermentation Industry)
- Characteristics and Function of Raw-starch-affinity Site on Aspergillus awamori var. kawachi Glucoamylase I Molecule
- Structure of the Raw-starch-affinity Site on the Aspergillus awamori var. kawachi Glucoamylase I Molecule
- Regeneration of Protoplasts of Clostridium saccharoperbutylacetonicum
- Fermentation of Cellulose by a Newly Isolated Thermophilic Clostridium sp.
- Phage Taillike Particles Found in the Pocks of Streptomyces coerulescens
- Isolation of a Linear DNA Associated with Pock Formation in Streptomyces azureus
- Some Properties of Autolysin of Clostridium saccharoperbutylacetonicum
- Studies on Sucrose-Induced Autolysis of Clostridial Cells (Part 3) Autolytic Formation of Clostridial Protoplasts (Autoplasts) as Revealed by Microscopy and Their Some Properties
- Isolation and Characteristics of an Acetone-Butanol-negative, Ethanol-Isovaleric Acid-producing Mutant of Clostridium saccharoperbutylacetonicum N1-4 ATCC 13564(Microbiology & Fermentation Industry)
- Degeneration of Solventogenic Clostridium Caused by a Defect in NADH Generation(Microbiology & Fermentation Industry)
- Metabolic mechanism of Ethanol-Isovaleric Acid Fermentation by a Clostridium saccharoperbutylacetonicum UV-Mutant(Microbiology & Fermentation Industry)
- Isolation and Antitumor Activity of a New Rhamnose Polysaccharide, Clostrhamman from Clostridium saccharoperbutylacetonicum
- Silicified Microbial Community at Steep Cone Hot Spring,Yellowstone National Park
- Effect of Thermophlilic Bacteria on the Siliceous Deposition and Phlylogenetic Analysis of the Bacterial Diversity in Silica Scale
- Effects of UV Dose on Formation of Spontaneously Developing Pocks in Streptomyces azureus ATCC14921(Microbiology & Fermentation Technology)
- Characterization of a Phage Resistance Plasmid, pLKS, of Silage-Making Lactobacillus plantarum NGRI0101
- A Giant Phage Taillike Particle of Clostridium Saccharoperbutylacetonicum ATCC 13564
- Mode of Action of Clostocin O Part 2. On the Synthesis of Nucleic Acid in Sensitive Bacteria
- Mode of Action of Clostocin O Part 1. On the Macromolecular Synthesis in Sensitive Bacteria
- Productions of Intracellular Melanoidindecolorizing Enzyme and Extracellular Antitumor Polysaccharide by Coriolus sp. No.20
- Antitumor Activity of Clostridial Cell Components
- Cloning and Nucleotide Sequence of γ-Polyglutamate Production Stimulating Factor on Bacillus subtilis (natto) Plasmid, pUH1
- Introduction of Kanamycin Resistant Gene to Pock-forming PlasmidpSA1.1 of Streptomyces azureus and Identification of spoIIIE-like Genein the Recombinant Plasmid
- Effects of Temperature on Escherichia coli β-Galactosidase Expression in Baculovirus-Insect Cell System
- Sporulation-Inhibitory Gene in Pock-forming Plasmid pSA1.1 of Streptomyces azureus
- Stimulation of Spore Formation, Mycelial Growth and Thiostrepton Production by several Thiol Compounds and β-NAD in Streptomyces azureus ATCC 14921 and Its Derivatives
- Heterologous Protein Production in Baculovirus-Insect Cell System
- Escherichia coli β-Galactosidase Production by Baculovirus-insect Cell System
- Sequence Analysis of Replication Origin of Plasmid pLS11 of Bacillus subtilis IFO 3022
- Molecular Cloning of Nucleotide Sequence of a Neutral Cellulase Gene of Thermophilic Clostridium sp.F-3
- Colorimetric Detection of DNA-DNA Hybridization in Microdilution Wells for Taxonomic Application on Bacterial Strains
- Inhibition of Spore Formation and Thiostrepton Production by Accumulated Intermediates of Purine Biosynthesis and the Suppression by AICA (5-Amino-4-Imidazole Carboxamide) in Purine Auxotrophic Mutants of Streptomyces azureus ATCC 14921
- Characterization of a Mutant of Thiostrepton-Producing Streptomyces azureus ATCC 14921 and Its Plasmids
- Changes in the Population of a Wide Host Range Actinophage Isolated from Thai Soil and Host Streptomycetes in Thai and Japanese Soil
- Bio-deposition of Amorphous Silica by an Extremely Thermophilic Bacterium, Thermus spp.
- Effects of Bacitracin and Excess Mg^ on Submerged Mycelial Growth of Streptomyces azureus
- Studies on Sucrose-Induced Autolysis of Clostridial Cells Part 2. Properties of Sucrose-Induced Autolysis
- Studies on Sucrose-Induced Autolysis of Clostridial Cells Part 1. Induction of a Rapid Bacterial Autolysis by Sucrose Treatment
- A Conjugative Linear Plasmid in Streptomyces laurentii ATCC31255
- Formation of Spontaneously Developing Pocks Regulated by Two Unhomologous Plasmids in Streptomyces laurentii
- Two Plasmids Related to Spontaneously Developing Pocks in Streptomyces laurentii
- Biological and Molecular Investigations of Spontaneously Developing Pocks in Streptomyces
- Protoplast Regeneration and Transformation of Thiostrepton-Producing Streptomyces laurentii ATCC 31255
- Whole Sequence of spoIIIE-Like, Sporulation-inhibitory, and Transfer Gene (spi) in a Conjugative Plasmid, pSA1.1,of Streptomyces azureus and Detection of spi-Like Gene in the Actinomycete Chromosome
- Successive Protoplast Transformation of Bacillus subtilis by Plasmid DNA under Low Concentration of Lysozyme
- Transfer Function of spi Gene in Plasmid pSA1.1 of Streptomyces azureus ATCC14921