Metabolic mechanism of Ethanol-Isovaleric Acid Fermentation by a Clostridium saccharoperbutylacetonicum UV-Mutant(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
Mutant AS2-1, derived from Clostridium saccharoperbutylacetonicum N1-4 ATCC 13564, did not produce acetone, butanol, and acetic acid, but had enhanced ethanol production and newly produced isovaleric acid at the molar ratio of 1:1. The mutant was defective in thiolase (acetyl-CoA acetyltransferase) activity, while the parent N1-4 showed high thiolase activity (103.5units/g protein). Addition of allyl alcohol into the mutant culture significantly decreased the production of both ethanol and isovaleric acid. AS2-1 mutant, therefore, had NAD^+- and NAD(P)^+-dependent aidehyde dehydrogenase on the pathway to ethanol and isovaleric acid, respectively. It was suggested that an excess of electrons accumulated as the result of the deficiency in thiolase was consumed for the reductive formation of isovalerate from pyruvate. The equation of the novel ethanol-isovaleric acid fermentation was concluded as 1.5mol glucose=1mol ethanol+1mol isovaleric acid+2mol CO_2+2mol H_2O+1mol H_2.
- 社団法人日本農芸化学会の論文
- 1990-02-23
著者
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Hayashida Shinsaku
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kyushu University
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Ahn Byoung
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kyushu University
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