Isolation and Characteristics of an Acetone-Butanol-negative, Ethanol-Isovaleric Acid-producing Mutant of Clostridium saccharoperbutylacetonicum N1-4 ATCC 13564(Microbiology & Fermentation Industry)
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概要
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A mutant, AS2-1, was isolated by UV irradiation from Clostridium saccharoperbutylacetonicum N1-4 ATCC 13564 producing hyper butanol at the molar ratio of acetone-butanol-ethanol (3.3 : 9.5 : 1). The mutant proved to be deficient in the production of acetone, butanol, and acetic acid, but enhanced the ethanol (17.7 times increase) and newly produced isovaleric acid at a molar ratio of 1:1. The maximum production of ethanol and isovaleric acid reached 10.6g/l(0.23M) and 19.8g/l(0.20M) from glucose at 77.7g/l(0.43M) in TYA medium. As the equimolar ratio of ethanol and isovaleric acid was also produced in an amino acid-free minimal medium containing ammonium acetate as a nitrogen source, isovaleric acid was not produced from amino acids, but de novo synthesized from the ammonium and glucose.
- 社団法人日本農芸化学会の論文
- 1990-02-23
著者
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Hayashida Shinsaku
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kyushu University
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Ahn B
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kyushu University
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Ahn Byoung
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kyushu University
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