Degeneration of Solventogenic Clostridium Caused by a Defect in NADH Generation(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
A degenerated strain of Clostridium saccharoperbutylacetonicum N1-4 ATCC13564 was isolated after 30 subcultures and was designated as DGN32. The product yields of DGN32 drastically decreased to below one-third of those of N 1-4, but its acetone productivity remained the same as that of N1-4 when the culture pH was at 5.1. DGN32 with or without pH control evolved almost equimolar amounts of CO_2 and H_2 throughout the fermentation. The amount of NADH consumed for the acid and solvent production of DGN32 corresponded to that of NADH generated in the EMP pathway. These results suggested that the electron flow coupled with pyruvate metabolism to acetyl-CoA in DGN32 would be kept for H_2 evolution, but not for NADH generation. However DGN32 cultivated with a redox dye (benzyl viologen) preferentially produced butanol though the butanol production rate was comparatively low. In DGN32 there was suppression of the NADH generation system coupled with pyruvate metabolism to acetyl-CoA, which could be essential for the metabolic transition from the acidogenic phase to the solventogenic phase.
- 社団法人日本農芸化学会の論文
- 1990-02-23
著者
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Hayashida Shinsaku
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kyushu University
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YOSHINO Sadazo
Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Kyushu University
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Yoshino Sadazo
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kyushu University
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