Kinetic modeling and sensitivity analysis of xylose metabolism in Lactococcus lactis IO-1(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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概要

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• We proposed a kinetic simulation model of xylose metabolism in Lactococcus lactis IO-1 that describes the dynamic behavior of metabolites using the simulator WinBEST-KIT. This model was developed by comparing the experimental time-course data of metabolites in batch cultures grown in media with initial xylose concentrations of 20.3-57.8g/l with corresponding calculated data. By introducing the terms of substrate activation, substrate inhibition, and product inhibition, the revised model showed a squared correlation coefficient (r^2) of 0.929 between the experimental time-course of metabolites and the calculated data. Thus, the revised model is assumed to be one of the best candidates for kinetic simulation describing the dynamic behavior of metabolites. Sensitivity analysis revealed that pyruvate flux distribution is important for higher lactate production. To confirm the validity of our kinetic model, the results of the sensitivity analysis were compared with enzyme activities observed during increasing lactate production by adding natural rubber serum powder to the xylose medium. The experimental results on pyruvate flux distribution were consistent with the prediction by sensitivity analysis.

• 社団法人日本生物工学会の論文

著者

• SEKIGUCHI Tatsuya

  Department of Mechanical Engineering, Faculty of Science and Engineering, Ritsumeikan University

• OKAMOTO Masahiro

  Laboratory of Exercise Biochemistry, University of Tsukuba Graduate School of Comprehensive Human Sc

• Sonomoto Kenji

  Lab. Of Microbial Technol. Div. Of Microbial Sci. And Technol. Dep. Of Bioscience And Biotechnology

• Sonomoto K

  (present Address)department Of Biochemical Engineering And Science Faculty Of Computer Science And S

• Sonomoto Kenji

  Lab. Of Functional Food Design Dep. Of Functional Metabolic Design Bio-architecture Center Kyushu Un

• Sonomoto Kenji

  Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyoto University:(

• Sonomoto Kenji

  Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci

• Sonomoto Kenji

  Laboratory Of Microbial Technology Department Of Food Science And Technology Faculty Of Agriculture

• Sonomoto Kenji

  Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci

• ISHIZAKI Ayaaki

  Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bios

• Sonomoto Kenji

  Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci

• Sonomoto Kenji

  Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot

• Ishizaki Ayaaki

  Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci

• Ishizaki Ayaaki

  Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture

• Ishizaki Ayaaki

  Department Of Food Science And Technology Faculty Of Agriculture Kyushu University

• Ishizaki Ayaaki

  Division Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Science

• Ishizaki Ayaaki

  Laboratory Of Microbial Science And Technology Division Of Bioscience And Biotechnology Graduate Sch

• Ishizaki Ayaaki

  Laboratory Of Microbial Technology Department Of Food Science And Technology Faculty Of Agriculture

• Sonomoto Kenji

  Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture

• Shinto Hideaki

  Laboratory of Microbial Technology, Department of Bioscience and Biotechnology, Faculty of Agricultu

• Ishizaki A

  Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture

• Shinto Hideaki

  Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture

• Oshiro Mugihito

  Laboratory of Microbial Technology, Department of Bioscience and Biotechnology, Faculty of Agricultu

• Tashiro Yukihiro

  Department of Life Study, Seinan Jo Gakuin University Junior College

• Miwa Noriko

  Laboratory of Microbial Technology, Department of Bioscience and Biotechnology, Faculty of Agricultu

• Miwa Noriko

  Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture

• Sekiguchi Tatsuya

  Department Of Information Engineering Faculty Of Engineering Maebashi Institute Of Technology

• Sonomoto Kenji

  Lab. Of Functional Food Design Dep. Of Functional Metabolic Design Bio-architecture Center Kyushu Un

• Tashiro Yukihiro

  Department Of Life Study Seinan Jo Gakuin University Junior College

• Oshiro Mugihito

  Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture

• Okamoto Masahiro

  Laboratory For Bioinformatics Graduate School Of Systems Life Science Kyushu University:laboratory O

• Tashiro Yukihiro

  Department of Bioscience and Biotechnology, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University

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