Complete Covalent Structure of Nisin Q, New Natural Nisin Variant, Containing Post-Translationally Modified Amino Acids
スポンサーリンク
概要
- 論文の詳細を見る
The third member of the nisin variant, nisin Q, produced by Lactococcus lactis 61-14, is a ribosomally-synthesized antimicrobial peptide, the so-called lantibiotic containing post-translationally modified amino acids such as lanthionine and dehydroalanine. Here, we determined the complete covalent structure of nisin Q, consisting of 34 amino acids, by two-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy. Sequential assignment of nisin Q containing the unusual amino acids was performed by total correlation spectroscopy (TOCSY) and nuclear Overhauser enhancement spectroscopy (NOESY). The observed long range nuclear Overhauser effect (NOE) in nisin Q indicated assignment of all five sets of lanthionines that intramolecularly bridge residues 3–7, 8–11, 13–19, 23–26, and 25–28. Consequently, the covalent structure of nisin Q was determined to hold the same thioether linkage formation as the other two nisins, but to harbor the four amino acid substitutions, in contrast with nisin A.
- 社団法人 日本農芸化学会の論文
著者
-
Obita Takayuki
Div. Of Medical Inst. Of Bioregulation Kyushu Univ.
-
Nakayama Jiro
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
-
Sonomoto Kenji
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
-
FUKAO Masanori
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bios
-
OBITA Takayuki
Division of Medical Institute of Bioregulation, Kyushu University
-
YONEYAMA Fuminori
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bios
-
KOHDA Daisuke
Division of Medical Institute of Bioregulation, Kyushu University
-
ZENDO Takeshi
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bios
関連論文
- 3P-1111 Enhanced Nukacin D13E Production in Lactococcus lactis NZ9000 by Overexpression of Immunity Genes
- Structures and Biosynthesis of Aflastatins: Novel Inhibitors of Aflatoxin Production by Aspergillus parasiticus
- The correlation between GI-tract microbiota and allergy in Japanese infants by molecular analysis
- Molecular Monitoring of the Developmental Bacterial Community in the Gastrointestinal Tract of Japanese Infants
- A Novel Lantibiotic, Nukacin ISK-1,of Staphylococcus warneri ISK-1 : Cloning of the Structural Gene and Identification of the Structure
- Calculation of Metabolic Flow of Xylose in Lactococcus lactis(METHOD)
- Xylooligosaccharide Fermentation with Leuconostoc lactis(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Nucleotide Sequences of Genes Encoding Allosamidin-sensitive and -insensitive Chitinases Produced by Allosamidin-producing Streptomyces(Biochemistry & Molecular Biology)
- Blasticidin A as an Inhibitor of Aflatoxin Production by Aspergillus parasiticus
- Isolation and Structure of staph-cAM373 Produced by Staphylococcus aureus That Induces Conjugal Transfer of Enterococcus faecalis Plasmid pAM373
- 乳酸菌ゲノムにコードされるクオーラムセンシングに関与する三分子制御系の遺伝子解析
- Complete Covalent Structure of Nisin Q, New Natural Nisin Variant, Containing Post-Translationally Modified Amino Acids
- Class IIa Bacteriocins from Lactic Acid Bacteria : Antibacterial Activity and Food Preservation
- Novel High-Efficient Butanol Production from Butyrate by Non-Growing Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) with Methyl Viologen(BIOCHEMICAL ENGINEERING)
- Pigment Synthesis by Immobilized Cultured Cells of Lavandula vera and Characterization of a Component of the Pigments(Microbiology & Fermentation Industry)
- Influence of Carbon Source on Pigment Production by Immobilized Cultured Cells of Lavandula vera
- Immobilized Plant Cell Reactor for Continuous Production of Blue Pigments(Microbiology & Fermentation Industry)
- Enhancement of Pigment Productivity of Immobilized Cultured Lavandula vera Cells by Limitation of Nitrogen Sources
- ATP-dependent leader peptide cleavage by NukT, a bifunctional ABC transporter, during lantibiotic biosynthesis(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Molecular Chaperones in Lactic Acid Bacteria : Physiological Consequences and Biochemical Properties
- Effect of a Negatively Charged Lipid on Membrane-Lacticin Q Interaction and Resulting Pore Formation
- A Novel Type of Immunity Protein, NukH, for the Lantibiotic Nukacin ISK-1 Produced by Staphylococcus warneri ISK-1
- Localization and Interaction of the Biosynthetic Proteins for the Lantibiotic, Nukacin ISK-1
- Purification and Identification of a Growth-Stimulating Peptide for Bifidobacterium bifidum from Natural Rubber Serum Powder
- Complementary Effects of Bifidogenic Growth Stimulators and Ammonium Sulfate in Natural Rubber Serum Powder on Bifidobacterium bifidum
- Utilization of Fermented Barley Extract Obtained from a By-product of Barley Shochu for Nisin Production(BREWING AND FOOD TECHNOLOGY)
- Lantibiotics : Diverse activities and unique modes of action
- Reconstitution and Function of Tetragenococcus halophila Chaperonin 60 Tetradecamer(Genetics, Molecular Biology, and Gene Engineering)
- Effect of Heterologous Expression of Molecular Chaperone DnaK from Tetragenococcus halophilus on Salinity Adaptation of Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- The groESL Operon of the Halophilic Lactic Acid Bacterium Tetragenococcus halophila(Microbiology & Fermentation Technology)
- Molecular Characterization and Regulatory Analysis of dnaK Operon of Halophilic Lactic Acid Bacterium Tetragenococcus halophila
- Cloning and Characterization of dnaK Operon of Tetragenococcus halophila
- Purification and Gene Cloning of Catalase from Staphylococcus warneri ISK-1
- Kinetic modeling and sensitivity analysis of xylose metabolism in Lactococcus lactis IO-1(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Utilization of Excess Sludge by Acetone-Butanol-Ethanol Fermentation Employing Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)(Biochemical Engineering)
- High Butanol Production by Clostridium saccharoperbutylacetonicum N1-4 in Fed-Batch Culture with pH-Stat Continuous Butyric Acid and Glucose Feeding Method(BIOCHEMICAL ENGINEERING)
- Characterization of a Gene Cluster of Staphylococcus warneri ISK-1 Encoding the Biosynthesis of and Immunity to the Lantibiotic, Nukacin ISK-1
- Synchronized Fresh Cell Bioreactor System for Continuous L-(+)-Lactic Acid Production Using Lactococcus lactis IO-1 in Hydrolysed Sago Starch
- pH-Dependent Continuous Lactic Acid Fermentation by Lactococcus lactis IO-1 Using Hydrolyzed Sago Starch.
- Catalase of Staphylococcus warneri ISK-1 Isolated from Nukadoko
- Extractive Acetone-Butanol-Ethanol Fermentation Using Methylated Crude Palm Oil as Extractant in Batch Culture of Clostridium saccharoperbutylacetonicum N1-4(ATCC 13564)
- Purification and Partial Identification of Bacteriocin ISK-1,a New Lantibiotic Produced by Pediococcus sp. ISK-1
- Nisin Z Production by Lactococcus lactis IO-1 Using Xylose as a Carbon Source
- A Bacteriocin of Strain Pediococcus sp. ISK-1 Isolated from Nukadoko, Bed of Fermented Rice Bran
- Chemical Synthesis and Biological Activity of The Gelatinase Biosynthesis-Activating Pheromone of Enterococcus faecalis and Its Analogs(Organic Chemistry)
- Identification of the Nukacin KQU-131, a New Type-A(II) Lantibiotic Produced by Staphylococcus hominis KQU-131 Isolated from Thai Fermented Fish Product (Pla-ra)
- Characterization and Structure Analysis of a Novel Bacteriocin, Lacticin Z, Produced by Lactococcus lactis QU 14
- Identification of the Lantibiotic Nisin Q, a New Natural Nisin Variant Produced by Lactococcus lactis 61-14 Isolated from a River in Japan(Microbiology & Fermentation Technology)
- Lantibiotics : Insight and Foresight for New Paradigm
- Heterologous Expression and Functional Analysis of the Gene Cluster for the Biosynthesis of and Immunity to the Lantibiotic, Nukacin ISK-1(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Growth of Curvularia lunata Spores into Mycelial Form within Various Gels, and Steroid 11β-Hydrooxylation by the Entrapped Mycelia
- Crystallographic and Functional Analyses of J-Domain of JAC1 Essential for Chloroplast Photorelocation Movement in Arabidopsis thaliana
- Kinetic Study on Δ^1-Dehydrogenation of Hydrocortisone by Gel-Entrapped Arthrobacter simplex Cells
- Effect of a Negatively Charged Lipid on Membrane-Lacticin Q Interaction and Resulting Pore Formation
- Identification of the Nukacin KQU-131, a New Type-A(II) Lantibiotic Produced by Staphylococcus hominis KQU-131 Isolated from Thai Fermented Fish Product (Pla-ra)
- Complete Covalent Structure of Nisin Q, New Natural Nisin Variant, Containing Post-Translationally Modified Amino Acids
- In Vivo and in Vitro Complementation Study Comparing the Function of DnaK Chaperone Systems from Halophilic Lactic Acid Bacterium Tetragenococcus halophilus and Escherichia coli
- Characterization and Structure Analysis of a Novel Bacteriocin, Lacticin Z, Produced by Lactococcus lactis QU 14
- Improvement of Heat Stability of Yeast Lipase by Chemical Modification with a Heterobifunctional Photogenerated Reagent
- ATP binding domain of NukT, a class-II lantibiotic transporter, is important for its peptidase activity
- Antibacterial Peptides "Bacteriocins" : An Overview of Their Diverse Characteristics and Applications
- Identification of Enterocin NKR-5-3C, a Novel Class IIa Bacteriocin Produced by a Multiple Bacteriocin Producer, Enterococcus faecium NKR-5-3
- Purification and Characterization of Multiple Bacteriocins and an Inducing Peptide Produced by Enterococcus faecium NKR-5-3 from Thai Fermented Fish
- Monitoring of the multiple bacteriocin production by Enterococcus faecium NKR-5-3 through a developed liquid chromatography and mass spectrometry-based quantification system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Up to Species-level Community Analysis of Human Gut Microbiota by 16S rRNA Amplicon Pyrosequencing
- High-Throughput Screening of Inhibitors Targeting Agr/Fsr Quorum Sensing in Staphylococcus aureus and Enterococcus faecalis