Heterologous Expression and Functional Analysis of the Gene Cluster for the Biosynthesis of and Immunity to the Lantibiotic, Nukacin ISK-1(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
- 論文の詳細を見る
Nukacin ISK-1 is a lantibiotic produced by Staphylococcus warneri ISK-1. The gene cluster of nukacin ISK-1 consists of at least nukAMTFEG, ORF1 and ORF7. In this study, we demonstrated the heterologous production of nukacin ISK-1 in Lactococcm lactis by the artificial polycistronic expression of nukAMTFEG-ORFl under the control of the nisin-controlled expression (NICE) system. Consequently, the recombinant L. lactis showed antimicrobial activity. Mass analysis clarified the presence of nukacin ISK-1 produced in the culture supernatant. These results suggested that the recombinant L. lactis produced nukacin ISK-1 heterologously. Inactivation of nukA, -M or -T resulted in the complete loss of the nukacin ISK-1 production phenotype. This finding suggested that nukAMT are indispensably associated with the biosynthesis of nukacin ISK-1. To our knowledge, this is the first report of the heterologous production of lantibiotic using the NICE system.
- 社団法人日本生物工学会の論文
- 2004-12-25
著者
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Aso Y
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Nakayama J
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Nakayama Jiro
Lab. Of Microbial Technol. Div. Of Microbial Sci. And Technol. Dep. Of Bioscience And Biotechnology
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Nakayama Jiro
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Nakayama Jiro
Department Of Applied Biological Chemistry Graduate School Of Agriculture And Life Sciences The Univ
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Nagao Jun-ichi
Dep. Of Functional Bioscience Section Of Infection Biology Fukuoka Dental Coll. 2-15-1 Tamura Sawara
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Nagao Jun-ichi
Department Of Functional Bioscience Section Of Infection Biology Fukuoka Dental College
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Sonomoto Kenji
Lab. Of Microbial Technol. Div. Of Microbial Sci. And Technol. Dep. Of Bioscience And Biotechnology
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Sonomoto K
(present Address)department Of Biochemical Engineering And Science Faculty Of Computer Science And S
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Sonomoto Kenji
Lab. Of Functional Food Design Dep. Of Functional Metabolic Design Bio-architecture Center Kyushu Un
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Sonomoto Kenji
Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyoto University:(
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Sonomoto Kenji
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Sonomoto Kenji
Laboratory Of Microbial Technology Department Of Food Science And Technology Faculty Of Agriculture
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Sonomoto Kenji
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Kimura H
Kagawa Nutrition Univ. Tokyo Jpn
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SASHIHARA Toshihiro
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bios
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Sonomoto Kenji
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Sonomoto Kenji
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Sonomoto Kenji
Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture
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Aso Yuji
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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OKUDA Ken-ichi
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bios
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NAGAO Jun-ichi
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bios
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KANEMASA Youhei
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bios
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KOGA Hanako
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bios
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Koga H
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Kanemasa Youhei
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Kimura Hiromi
Laboratory Of Microbial Technology Department Of Food Science And Technology Faculty Ofagriculture K
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Okuda Ken-ichi
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Sashihara Toshihiro
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Sonomoto Kenji
Lab. Of Functional Food Design Dep. Of Functional Metabolic Design Bio-architecture Center Kyushu Un
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Nagao Jun-ichi
Laboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
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