Development of Purification Method and Identification of a Peptide Antibiotic Produced by Lactococcus lactis IO-1.
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概要
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The culture supernatant of <I>Lactococcus lactis</I> IO-1, which was isolated in our laboratory, inhibited the cell growth of various Gram-positive bacteria but did not inhibit the nisin A-producing strain, <I>L. lactis</I> NCDO 497. A nisin-like peptide antibiotic produced by <I>L. lactis</I> IO-1 was efficiently purified sequentially by acid treatment (at pH 3), ammonium sulfate precipitation, cation-exchange chromatography and reversed-phase high performance liquid chromatography. Dissociation of the peptide aggregates with high molar concentrations of urea resulted in successful purification. The molecular mass of this peptide antibiotic was 3335.67 by fast atom bombardment-mass spectrometry, confirming that the peptide antibiotic from <I>L. lactis</I> IO-1 is nisin Z, a natural nisin variant. The purification method used is rapid, simple and effective, permitting the specific activity to increase 122-fold, and the recovery was 24%.
- 社団法人 日本食品科学工学会の論文
著者
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Sonomoto Kenji
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Ishizaki Ayaaki
Laboratory Of Microbial Science And Technology Division Of Bioscience And Biotechnology Graduate Sch
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Endo Naoyuki
Laboratory of Microbial Technology, Department of Food Science and Technology, Faculty of Agricultur
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Endo Naoyuki
Laboratory of Microbial Technology, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University
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ISHIZAKI Ayaaki
Laboratory of Microbial Technology, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University
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MATSUSAKI Hiromi
Laboratory of Microbial Technology, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University
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SONOMOTO Kenji
Laboratory of Microbial Technology, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University
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