Purification and Identification of a Growth-Stimulating Peptide for Bifidobacterium bifidum from Natural Rubber Serum Powder
スポンサーリンク
概要
- 論文の詳細を見る
Natural rubber serum powder, which is a by-product obtained in the production of latex rubber, has a strong growth-stimulating activity for Bifidobacterium bifidum JCM 1254. The retained fraction obtained by ultrafiltration (molecular weight cutoff 1000) showed a growth-stimulating activity in a dose-dependent manner on B_<12> assay medium with ammonium sulfate. One of the growth stimulators was purified from the retained fraction by acetone precipitation, solid-phase extraction with a hydrophobic pretreatment column, and multistage reversed-phase HPLC. An increase of 53-fold in the specific activity, and a recovery of 1.3% were obtained. The amino acid composition and N-terminal sequence analysis of this growth stimulator provided the structure of Ala-Thr-Pro-Glu-Lys-Glu-Glu-Pro-Thr-Ala. The molecular mass was 1075 by MALDI-TOF MS analysis. These results showed that this growth stimulator was a decapeptide with the sequence shown above. This is the first report that clarified the structure of an active peptide for the growth of Bifidobacterium.
- 社団法人日本農芸化学会の論文
- 2000-10-23
著者
-
Sonomoto K
(present Address)department Of Biochemical Engineering And Science Faculty Of Computer Science And S
-
Sonomoto Kenji
Lab. Of Functional Food Design Dep. Of Functional Metabolic Design Bio-architecture Center Kyushu Un
-
Sonomoto Kenji
Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyoto University:(
-
Sonomoto Kenji
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
-
Sonomoto Kenji
Laboratory Of Microbial Technology Department Of Food Science And Technology Faculty Of Agriculture
-
Sonomoto Kenji
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
-
ISHIZAKI Ayaaki
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bios
-
Sonomoto Kenji
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
-
Ishizaki Ayaaki
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
-
Ishizaki Ayaaki
Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture
-
Ishizaki Ayaaki
Department Of Food Science And Technology Faculty Of Agriculture Kyushu University
-
Ishizaki Ayaaki
Division Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Science
-
Ishizaki Ayaaki
Laboratory Of Microbial Science And Technology Division Of Bioscience And Biotechnology Graduate Sch
-
Ishizaki Ayaaki
Laboratory Of Microbial Technology Department Of Food Science And Technology Faculty Of Agriculture
-
Sonomoto Kenji
Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture
-
Ishizaki A
Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture
-
Etoh S
Laboratory Of Microbial Technology Division Of Microbial Science And Technology.department Of Biosci
-
Asamura Kayoko
Laboratory of Microbial Technology, Department of Food Science and Technology,Faculty of Agriculture
-
Obu Azumi
Laboratory of Microbial Technology, Department of Food Science and Technology,Faculty of Agriculture
-
ETOH Shinichi
Laboratory of Microbial Science and Technology, Division of Bioscience and Biotechnology, Graduate S
-
Obu Azumi
Laboratory Of Microbial Science And Technology Division Of Bioscience And Biotechnology Graduate Sch
-
Asamura Kayoko
Laboratory Of Microbial Science And Technology Division Of Bioscience And Biotechnology Graduate Sch
-
Etoh Shin-ichi
Laboratory Of Microbial Technology Division Of Microbial Science And Technology.department Of Biosci
関連論文
- 3P-1111 Enhanced Nukacin D13E Production in Lactococcus lactis NZ9000 by Overexpression of Immunity Genes
- The correlation between GI-tract microbiota and allergy in Japanese infants by molecular analysis
- Molecular Monitoring of the Developmental Bacterial Community in the Gastrointestinal Tract of Japanese Infants
- A Novel Lantibiotic, Nukacin ISK-1,of Staphylococcus warneri ISK-1 : Cloning of the Structural Gene and Identification of the Structure
- Calculation of Metabolic Flow of Xylose in Lactococcus lactis(METHOD)
- Xylooligosaccharide Fermentation with Leuconostoc lactis(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- pH-Mediated Control Methods for Continuous Ethanol Fermentation Using Zymomonas mobilis
- Complete Covalent Structure of Nisin Q, New Natural Nisin Variant, Containing Post-Translationally Modified Amino Acids
- Class IIa Bacteriocins from Lactic Acid Bacteria : Antibacterial Activity and Food Preservation
- Novel High-Efficient Butanol Production from Butyrate by Non-Growing Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) with Methyl Viologen(BIOCHEMICAL ENGINEERING)
- Development of a Simple Non-Freezing Method to Preserve Cultured Plant Cells
- Pigment Synthesis by Immobilized Cultured Cells of Lavandula vera and Characterization of a Component of the Pigments(Microbiology & Fermentation Industry)
- Influence of Carbon Source on Pigment Production by Immobilized Cultured Cells of Lavandula vera
- Immobilized Plant Cell Reactor for Continuous Production of Blue Pigments(Microbiology & Fermentation Industry)
- Enhancement of Pigment Productivity of Immobilized Cultured Lavandula vera Cells by Limitation of Nitrogen Sources
- ATP-dependent leader peptide cleavage by NukT, a bifunctional ABC transporter, during lantibiotic biosynthesis(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Molecular Chaperones in Lactic Acid Bacteria : Physiological Consequences and Biochemical Properties
- Effect of a Negatively Charged Lipid on Membrane-Lacticin Q Interaction and Resulting Pore Formation
- A Novel Type of Immunity Protein, NukH, for the Lantibiotic Nukacin ISK-1 Produced by Staphylococcus warneri ISK-1
- Localization and Interaction of the Biosynthetic Proteins for the Lantibiotic, Nukacin ISK-1
- Purification and Identification of a Growth-Stimulating Peptide for Bifidobacterium bifidum from Natural Rubber Serum Powder
- Complementary Effects of Bifidogenic Growth Stimulators and Ammonium Sulfate in Natural Rubber Serum Powder on Bifidobacterium bifidum
- Utilization of Fermented Barley Extract Obtained from a By-product of Barley Shochu for Nisin Production(BREWING AND FOOD TECHNOLOGY)
- Lantibiotics : Diverse activities and unique modes of action
- Reconstitution and Function of Tetragenococcus halophila Chaperonin 60 Tetradecamer(Genetics, Molecular Biology, and Gene Engineering)
- Effect of Heterologous Expression of Molecular Chaperone DnaK from Tetragenococcus halophilus on Salinity Adaptation of Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- The groESL Operon of the Halophilic Lactic Acid Bacterium Tetragenococcus halophila(Microbiology & Fermentation Technology)
- Molecular Characterization and Regulatory Analysis of dnaK Operon of Halophilic Lactic Acid Bacterium Tetragenococcus halophila
- Cloning and Characterization of dnaK Operon of Tetragenococcus halophila
- Purification and Gene Cloning of Catalase from Staphylococcus warneri ISK-1
- Sequence Analysis of Replication Origin of Plasmid pLS11 of Bacillus subtilis IFO 3022
- Molecular Cloning of Nucleotide Sequence of a Neutral Cellulase Gene of Thermophilic Clostridium sp.F-3
- General Character and Taxonomic Study of Lactococcus lactis IO-1, JCM 7638
- Kinetic modeling and sensitivity analysis of xylose metabolism in Lactococcus lactis IO-1(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Utilization of Excess Sludge by Acetone-Butanol-Ethanol Fermentation Employing Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)(Biochemical Engineering)
- High Butanol Production by Clostridium saccharoperbutylacetonicum N1-4 in Fed-Batch Culture with pH-Stat Continuous Butyric Acid and Glucose Feeding Method(BIOCHEMICAL ENGINEERING)
- Characterization of a Gene Cluster of Staphylococcus warneri ISK-1 Encoding the Biosynthesis of and Immunity to the Lantibiotic, Nukacin ISK-1
- Synchronized Fresh Cell Bioreactor System for Continuous L-(+)-Lactic Acid Production Using Lactococcus lactis IO-1 in Hydrolysed Sago Starch
- Development of a Novel Method for Feeding a Mixture of L-Lactic Acid and Acetic Acid in Fed-Batch Culture of Ralstonia eutropha for Poly-D-3-Hydroxybutyrate Production
- pH-Dependent Continuous Lactic Acid Fermentation by Lactococcus lactis IO-1 Using Hydrolyzed Sago Starch.
- Catalase of Staphylococcus warneri ISK-1 Isolated from Nukadoko
- Optimization of L-Lactic Acid Feeding for the Production of Poly-K-3-Hydroxybutyric Acid by Alcaligenes eutrophus in Fed-Batch Culture
- Extractive Acetone-Butanol-Ethanol Fermentation Using Methylated Crude Palm Oil as Extractant in Batch Culture of Clostridium saccharoperbutylacetonicum N1-4(ATCC 13564)
- Purification and Partial Identification of Bacteriocin ISK-1,a New Lantibiotic Produced by Pediococcus sp. ISK-1
- Nisin Z Production by Lactococcus lactis IO-1 Using Xylose as a Carbon Source
- A Bacteriocin of Strain Pediococcus sp. ISK-1 Isolated from Nukadoko, Bed of Fermented Rice Bran
- Identification of the Nukacin KQU-131, a New Type-A(II) Lantibiotic Produced by Staphylococcus hominis KQU-131 Isolated from Thai Fermented Fish Product (Pla-ra)
- Characterization and Structure Analysis of a Novel Bacteriocin, Lacticin Z, Produced by Lactococcus lactis QU 14
- Identification of the Lantibiotic Nisin Q, a New Natural Nisin Variant Produced by Lactococcus lactis 61-14 Isolated from a River in Japan(Microbiology & Fermentation Technology)
- Lantibiotics : Insight and Foresight for New Paradigm
- Heterologous Expression and Functional Analysis of the Gene Cluster for the Biosynthesis of and Immunity to the Lantibiotic, Nukacin ISK-1(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Natural Rubber Serum Powder, an Enhancer for the Growth of Bifidobacterium
- Growth of Curvularia lunata Spores into Mycelial Form within Various Gels, and Steroid 11β-Hydrooxylation by the Entrapped Mycelia
- Formation of Heteroduplex Molecules between Plasmids pUH1 and pLS11 in Polyglutamate-producing Bacillus Strains
- Continuous Hydrolysis of Starch in Membrane Unit Connected Immobilized Enzyme Reactor
- Kinetic Study on Δ^1-Dehydrogenation of Hydrocortisone by Gel-Entrapped Arthrobacter simplex Cells
- 571 Computer-mediated addition of fresh medium in continuous culture of Zymomonas mobilis by the monitoring of weight changes
- Characterization of Newly Isolated Bacillus subtilis C1 Which Is Sensitive to Antibiotic Produced by Lactic Acid Coccus
- Effect of Chemical Modification of Tyrosine Residues on Activities of Bacterial Lipase
- Effect of a Negatively Charged Lipid on Membrane-Lacticin Q Interaction and Resulting Pore Formation
- Identification of the Nukacin KQU-131, a New Type-A(II) Lantibiotic Produced by Staphylococcus hominis KQU-131 Isolated from Thai Fermented Fish Product (Pla-ra)
- Complete Covalent Structure of Nisin Q, New Natural Nisin Variant, Containing Post-Translationally Modified Amino Acids
- In Vivo and in Vitro Complementation Study Comparing the Function of DnaK Chaperone Systems from Halophilic Lactic Acid Bacterium Tetragenococcus halophilus and Escherichia coli
- Characterization and Structure Analysis of a Novel Bacteriocin, Lacticin Z, Produced by Lactococcus lactis QU 14
- Improvement of Heat Stability of Yeast Lipase by Chemical Modification with a Heterobifunctional Photogenerated Reagent
- 525 Ethanol production by Zymomonas mobilis with cell recycling using a hollow fiber filter module
- Built-in Electrodialysis Batch Culture, a New Approach to Release of End Product Inhibition
- ATP binding domain of NukT, a class-II lantibiotic transporter, is important for its peptidase activity
- Reduction in the Length of the Lag Phase of L-Lactate Fermentation by the Use of Inocula from Electrodialysis Seed Cultures
- Studies on Dissolved Hydrogen Behavior in Autototrophic Culture of Alcaligenes eutrophus ATCC 17697T
- Development of a Dissolved Hydrogen Sensor and Its Application to Evaluation of Hydrogen Mass Transfer
- Accumulation of Polyphosphate and Substrate Gas Utilization Efficiency in PHB Accumulation Phase of Autotrophic Batch Culture of Alcaligenes eutrophus ATCC17697^T
- Investigation of the Utility of Pineapple Juice and Pineapple Waste Material as Low-Cost Substrate for Ethanol Fermentation by Zymomonas mobilis
- Ethanol Production by Zymomonas mobilis Using Natural Rubber Waste as a Nutritional Source
- 110 Effect of natural rubber waste on a batch culture ethanol formation using Zymomonas mobillis
- Optimization of the Culture Medium for Growth and the Kinetics of Lactate Fermentation by Pediococcus sp. ISK-1
- Comparative Study of Staphylococcal Catalases
- Molecular Cloning of a Thermophilic Alkaline Cellulase Gene in Escherichia coli(Microbiology & Fermentation Industry)
- Chemical Composition of Palm Fiber and Its Feasibility as Cellulosic Raw Material for Sugar Production(Food & Nutrition)
- Effects of Non-covalent Interaction of Dimethyl Suberimidate on Lipase Stability(Biological Chemistry)
- 540 Production of nisin Z from xylose by Lactococcus lactis IO-1
- Growth-Stimulation Effects of Natural Rubber Serum on Bifidobacterium bifidum
- Stimulation by Natural Rubber Serum Powder of the Growth of Bifidobacterium bifidum
- Purification and Identification of a Peptide Antibiotic Produced by Lactococcus lactis IO-1
- 238 Fermentative production of peptide antibiotic (nisin Z) by Lactococcus lactis IO-1 cells immobilized with various kinds of gel materials
- Antibacterial Peptides "Bacteriocins" : An Overview of Their Diverse Characteristics and Applications
- Identification of Enterocin NKR-5-3C, a Novel Class IIa Bacteriocin Produced by a Multiple Bacteriocin Producer, Enterococcus faecium NKR-5-3
- Purification and Characterization of Multiple Bacteriocins and an Inducing Peptide Produced by Enterococcus faecium NKR-5-3 from Thai Fermented Fish
- Monitoring of the multiple bacteriocin production by Enterococcus faecium NKR-5-3 through a developed liquid chromatography and mass spectrometry-based quantification system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Novel high butanol production from lactic acid and pentose by Clostridium saccharoperbutylacetonicum(BIOCHEMICAL ENGINEERING)
- Effect of Lactobacillus brevis 119-2 isolated from Tsuda kabu red turnips on cholesterol levels in cholesterol-administered rats(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Genomic Features of Lactococcus lactis IO-1, a Lactic Acid Bacterium That Utilizes Xylose and Produces High Levels of L-Lactic Acid
- Some Characteristics of Nisin Z, a Peptide Antibiotic Produced by Lactococcus lactis IO-1.
- Cholesterol-lowering Effects of Lactobacillus brevis Isolated from Turnip "Tsuda Kabu"
- Cholesterol-lowering Effects of Lactobacillus brevis Isolated from Turnip "Tsuda Kabu"
- Development of Purification Method and Identification of a Peptide Antibiotic Produced by Lactococcus lactis IO-1.
- Efficient butanol production without carbon catabolite repression from mixed sugars with Clostridium saccharoperbutylacetonicum N1-4(ENVIRONMENTAL BIOTECHNOLOGY)
- Up to Species-level Community Analysis of Human Gut Microbiota by 16S rRNA Amplicon Pyrosequencing
- High-Throughput Screening of Inhibitors Targeting Agr/Fsr Quorum Sensing in Staphylococcus aureus and Enterococcus faecalis
- Genomic Features of Lactococcus lactis IO-1, a Lactic Acid Bacterium That Utilizes Xylose and Produces High Levels of L-Lactic Acid