Bioconversions under Hydrophobic Conditions : Effect of Solvent Polarity on Steroid Transformations by Gel-entrapped Nocardia rhodocrous Cells
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概要
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Whole cells of Nocardia rhodocrous NCIB 10554 were immobilized by entrapment with photo-crossilinkable resin prepolymers or urethane prepolymers of either a hydropilic or hydrophobic nature, and their activities to convert 3β-hydroxy-Δ^5-steroids to the corresponding 3-keto-Δ^4-steroids in organic solvent systems were examined. In cholesterol transformation in a mixutre of chloroform and n-heptane (1 : 1 by volume), the activity of the cells entrapped in hydrophobic gels was higher than that of the hydrophilic gel-entrapment cells. In the case of pregnenolone transformation in chloroform and n-heptane, however, the activity of the latter cells was rather higher than that of the former. No difference in the activity was observed in transformation of dehydroepiandrosterone in chloroform and n-heptane between the hydrophobic gel-entrapped cells and hydrophilic gel-entrapped cells. These differences of gel hydrophobicity in the steroid conversions by the entrapped cells were ascribable to the differences in hydrophobicity of the steroids employed. The effect of the solvent polarity on the cholesterol transformation was examined in more detail using various organic solvents mixed with an equivolume of n-heptane. The celles entrapped in hydrophilic gels could not convert cholesterol at all in non-polar solvents, such as carbon tetrachloride, benzene and toluene, while moderate activity was observed in more polar solvent, such as chloroform and methylene chloride. Thus, the transformation of cholesterol, a highly lipophilic compound, by the immobilized cells was markedly affected by the polarity of reaction solvents as well as the hydrophobicity of gels used for entrapment.
- 公益社団法人日本生物工学会の論文
- 1980-08-25
著者
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TANAKA ATSUO
Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemist
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Fukui Saburo
Laboratory Of Industrial Biochemisryt Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Omata Tetsuo
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Tanaka Atsuo
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Tanaka Atsuo
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chamistr
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