Assay of Cephalosporin C with an Immobilized Microbial Cell Tube System
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概要
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Acetone-dried cells of Citrobacter freundii having cephalosporinase were easily and tightly immobilized to the inner surface of glass tubes by use of an emulsion-type of photo-crosslinkable resin prepolymer. The microbial cell tubes thus prepared were used in a continuous flow system to measure cephalosporin C by potentiometry. A good response was observed in the concentration range from 0.1mM to 4mM of cephalosporin C. This system was active on cephalosporin C, deacetylcephalosporin C and deacetoxycephalosporin C, indicating the possibility for assaying the total amount of cephalosporins in fermentation broth as cephalosporin C values. The enzyme in the microbial cell tubes was stable after repeated use in at least 200 assays.
- 社団法人 日本農芸化学会の論文
著者
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Itoh Nobuya
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Fukui Saburo
Laboratory Of Industrial Biochemisryt Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Tanaka Atsuo
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chamistr
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