Novel Function of Rare Catechin, Epigallocatechin-3-(3"-O-methyl)gallate, against Cold Injury in Primary Rat Hepatocytes(MISCELLANEOUS)
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概要
- 論文の詳細を見る
Epigallocatechin-3-(3"-O-methyl)gallate (EGCg-3"-OMe) is a rare component in green tea leaf and its bioactivity is hardly known. In this paper, we report that EGCg-3"-OMe has the function for cold preservation of primary rat hepatocytes. Con fluent primary cultured hepatocytes were suspended in a storage solution, culture medium or cell banker (CB). EGCg-3"-OMe was tested as a supplement in the storage solution together with a general cryoprotectant, dimethylsulfoxide (DMSO). After 24 h cold preservation of cells at 4℃ followed by 1 h rewarming, cell viability and urea-synthesizing activity, one of the most important liver functions, were measured. EGCg-3"-OMe dose-dependently maintained cell viability and this effect was equal to that of a commercial CB at the highest concentration. Cell viability was also maintained after a further 24 h incubation at 37℃ of the cold-preserved hepatocytes. Conversely, urea-synthesizing activity was dose-dependently reduced by EGCg-3"-OMe. Cell protection by EGCg-3"-OMe due to the decrease in metabolic activity in cold-preserved cells was suggested. The decreased hepatic function of cells caused by EGCg-3"-OMe was rescued after a further 24 h incubation of cells at 37℃.
- 社団法人日本生物工学会の論文
- 2003-12-25
著者
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近藤 昌夫
Graduate School Of Pharmaceutical Sciences Osaka University:department Of Pharmaceutics And Biopharm
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Kawase Masaya
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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近藤 昌夫
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Yagi Kiyohito
Graduate School of Pharmaceutical Sciences, Osaka University
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Yagi K
Graduate School Of Pharmaceutical Sciences Osaka University
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Yagi Kiyohito
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Yagi K
Osaka Univ. Osaka Jpn
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Kawase M
Nagahama Institute Of Bio-science And Technology
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Kawase M
Osaka Univ. Osaka Jpn
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Hara Yukihiko
Tokyo Food Techno Co. Ltd.
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Kara Yukihiko
Tokyo Food Techno Company Ltd
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KAGAYA Noritaka
Graduate School of Pharmaceutical Sciences, Osaka University
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KAMIYOSHI AKIKO
Division of Laboratory Animal Research, Research Center for Human and Environmental Sciences, Shinsh
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TAGAWA YOH-ICHI
Division of Laboratory Animal Research, Research Center for Human and Environmental Sciences, Shinsh
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SAIJO RYOYASU
Tokyo Food Techno Company, Ltd
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Kagaya Noritaka
Graduate School Of Pharmaceutical Sciences Osaka University
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Yagi Kiyohito
Department Of Biocehmical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Kamiyoshi Akiko
Department Of Organ Regeneration Shinshu University Graduate School Of Medicine
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Kawase Mitsuo
Ngk Insulators Ltd.
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Kawase M
Research And Development Center Kobayashi Pharmaceutical Co. Ltd.
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Kawase Masaya
Faculty Of Education Kagawa University
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Saijo Ryoyasu
Tokyo Food Techno Company Ltd
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Kamiyoshi Akiko
Department Of Organ Regeneration Shinshu University School Of Medicine
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