Characterization of the Mouse TFF1 (pS2) Gene Promoter Region
スポンサーリンク
概要
- 論文の詳細を見る
Trefoil peptides (TFTs) with a unique trefoil domain (s) are presumed to function in protection and repair of the gastrointestinal epithelial layer. Three peptide family members are differently distributed in the mouse gastrointestinal tract:TFF1/pS2 specifically in stomach, TFF2/SP mainly in stomach, pancreas and duodenum, and TFF3/ITF in intestine. We cloned and sequenced the mouse TFF1 gene 5'-upstream region by means of the genomic walking procedure. The cloned region was ligated to the luciferase reporter gene and then introduced into mouse gastric surface mucous GSM10 cells which express TFF1 and TFF2. The minimum promoter was located in the region containing the TATA-box between -39 and the transcriptional start site. Further upstream regions stimulated (-2192--1630bp, -641--243bp, -137--39bp) and inhibited (-1630--641bp, -243--137bp) luciferase gene expression. These regions as well as short segments conserved in the mouse and human 5'-upstream sequences may be important for modulation of the mRNA level of the TFF1 gene.
- 公益社団法人日本薬学会の論文
- 2001-02-01
著者
-
TABUCHI Yoshiaki
Molecular Genetics Research Center
-
TERADA Tomoyuki
Laboratory of Biochemistry, Faculty of Pharmacy, Osaka Ohtani University
-
Tabuchi Yoshiaki
Second Department Of Surgery Graduate School Of Medicine And Pharmaceutical Sciences University Of T
-
Terada Tomoyuki
Laboratory Of Biochemistry And Molecular Biology Graduate School Of Pharmaceutical Sciences Osaka Un
-
MAEDA Masatomo
Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka
-
Maeda Masatomo
Laboratory Of Biochemistry And Molecular Biology Graduate School Of Pharmaceutical Sciences Osaka Un
-
SAKAGAMI Reina
Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka
-
Maeda M
Dep. Of Molecular Biology School Of Pharmacy Iwate Medical Univ.
-
Sakagami Reina
Laboratory Of Biochemistry And Molecular Biology Graduate School Of Pharmaceutical Sciences Osaka Un
-
Terada Tomoyuki
Laboratory Of Biochemistry And Molecular Biology Graduate School Of Pharmaceutical Sciences Osaka Un
関連論文
- ウシ肝臓Recombinant dihydrodiol dehydrogenase(rDD3)の精製と諸性質
- ウシ肝臓Dihydrodiol dchydrogenase(DD3)のcDNAクローニングと発展
- Responses of Glutathione-Related Enzymes in Isolated Rat Small Intestine to Fe^-EDTA-Mediated Oxidative Stress
- Inhibition of Bovine Leukocyte Thioltransferase by Anti-inflammatory Drugs and Anti-histaminic Drugs
- 酸化的ストレスで活性化される遺伝子
- Response of osteoblasts and osteoclasts in regenerating scales to gravity loading
- DNA Microarray Analysis of Differentially Expressed Genes Responsive to Bisphenol A, an Alkylphenol Derivative, in an In Vitro Mouse Sertoli Cell Model
- Relationship between aging and dosage of warfarin : The current status of warfarin anticoagulant therapy for Japanese outpatients in a department of cardiovascular medicine
- Low-intensity ultrasound adjuvant therapy : enhancement of doxorubicin-induced cytotoxicity and the acoustic mechanisms involved
- Construction of artificial promoters sensitively responsive to sonication in vitro
- Confirmation of enhanced expression of heme oxygenase-1 gene induced by ultrasound and its mechanism : analysis by cDNA microarray system, real-time quantitative PCR, and Western blotting
- Characterization of a Novel Variant (S145C/L311V) of 3α-Hydroxysteroid/Dihydrodiol Dehydrogenase in Human Liver.
- Establishment and Characterization of a Colonic Epithelial Cell Line MCE301 from Transgenic Mice Harboring Temperature-Sensitive Simian Virus 40 Large T-Antigen Gene
- Development of a Conditionally Immortalized Testicular Sertoli Cell Line RTS3-3 from Adult Transgenic Rats Harboring Temperature-Sensitive Simian Virus 40 Large T-antigen Gene
- Development and Characterization of Conditionally Immortalized Gastric Epithelial Cell Lines from Transgenic Rats Harboring Temperature-Sensitive Simian Virus 40 Large T-antigen Gene
- Stable Expression of Gastric Proton Pump Activity at the Cell Surface
- Establishment and Characterization of Tracheal Epithelial Cell Lines, TM01 and TM02-3, from Transgenic Mice Bearing Temperature-Sensitive Simian Virus 40 Large T-Antigen Gene
- Establishment of gastric surface mucous cell lines from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene
- The Carboxyl Terminal Sequence of Rat Transporter Associated with Antigen Processing (TAP)-Like (ABCB9) is Heterogeneous due to Splicing of Its mRNA(Biochemistry/Molecular Biology)
- Significance of Lysine/Glycine Cluster Structure in Gastric H^+, K^+-ATPase
- Characterization of the Mouse TFF1 (pS2) Gene Promoter Region
- Membrane Localization of Transporter Associated with Antigen Processing (TAP)-Like (ABCB9) Visualized in Vivo with a Fluorescence Protein-Fusion Technique(Biochemistry/Molecular Biology)
- A Half-Type ABC TRansporter TAPL Is Highly Conserved between Rodent and Man, and the Human Gene Is Not Responsive to Interferon-γ in Contrast to TAP1 and TAP2^1
- Structures of P-type Transporting ATPases and Chromosomal Locations of Their Genes
- CHARACTERIZATON OF LENS GLUTATHIONE S-TRANSFERASES
- 3-Keto Reductase Activity of Estradiol-17β Dehydrogenase and Its Contribution to Androgen Metabolism
- HUMAN THIOLTRANSFERASE : ITS PURIFICATION AND PHYSIOLOGICAL ROLE
- Immortalized Gastric Epithelial Cell Line GSM06 Synthesizes Hyaluronan under the Influence of Simian Virus 40 Large T-Antigen Expression^1
- Interaction of Mat-8 (FXYD-3) with Na^+/K^+-ATPase in Colorectal Cancer Cells(Molecular and Cell Biology)
- Biochemical Characterization of Transporter Associated with Antigen Processing (TAP)-Like (ABCB9) Expressed in Insect Cells(Biochemistry)
- GATA-4 Gene Organization and Analysis of Its Promoter(Biochemistry)
- Further extension of mammalian GATA-6
- Characterization and Application of a Gastric Surface Mucous Cell Line GSM06 Established From Temperature-Sensiteve Simian Virus 40 Large T-Antigen Transgenic Mice
- Role of Glutathione S-Transferases in Lens under Oxidative Stress
- A Unique Role of an Amino Terminal 16-Residue Region of Long-Type GATA-6
- Stable Expression of Gastric Proton Pump Activity at the Cell Surface.