Membrane Localization of Transporter Associated with Antigen Processing (TAP)-Like (ABCB9) Visualized in Vivo with a Fluorescence Protein-Fusion Technique(Biochemistry/Molecular Biology)
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概要
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Transporter associated with antigen processing (TAP)-like (TAPL, ABCB9) is a half-type ATP binding cassette (ABC) protein belonging to subfamily B highly homologous to the TAP, a hetero-dimeric complex consisting of a TAP1 and a TAP2 subunit. Human TAPL, to which was tagged with green fluorescence protein (GFP) at its carboxyl terminus (TAPL-GFP), showed fluorescence on intracellular membranes similar to TAP1-GFP. A truncated form of TAPL-L-GFP (M^1-S^<275> was followed by GFP) showed a similar cellular fluorescence pattern to TAPL-GFP. However, the fluorescence of TAPL-S-GFP (M^1-G^<75>) was distributed over all the cellular membranes including plasma membrane, indicating that the amino terminal region of TAPL (M^1-S^<275>) is essential for its localization to the intracellular membranes. A co-expression study demonstrated that TAPL-S-GFP was co-localized with TAPL-DR (DsRed-tagged TAPL) or TAP1-DR, suggesting that TAPL is able to interact with not only itself but also with TAP1 through the M^1-G^<75> region of TAPL. It is also proposed that a further downstream sequence of TAPL would confine the distribution of TAPL-S-GFP to the intracellular membranes. Similarly, the distribution of TAP2-S-GFP (M^1-R^<88>) was restricted to the intracellular membranes by TAPL-DR or TAP1-DR, indicating that the M^1-R^<88> region of TAP2 is able to interact with TAPL as well as TAP1. Therefore, TAPL would form a homo-dimer with itself, and a hetero-dimer with TAP1 and TAP2. TAPL-GFP was co-localized with the fluorescence endoplasmic reticulum (ER) marker, suggesting that TAPL is mainly localized to the ER in the intracellular membranes.
- 公益社団法人日本薬学会の論文
- 2004-12-01
著者
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前田 光子
Life Science Center Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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Takagi Mutsumi
Pharmaceutical Research And Development Department Asahi Chemical Industry Company Ltd.:(present Add
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Maeda M
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Nagasaki University:faculty Of Pharmac
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Takagi M
Japan Advanced Inst. Sci. And Technol. Ishikawa Jpn
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KOBAYASHI Ayako
Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka
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MAEDA Masatomo
Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka
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Maeda Masatomo
Laboratory Of Biochemistry And Molecular Biology Graduate School Of Pharmaceutical Sciences Osaka Un
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MAEDA Tatsuo
Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka
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Maeda T
Laboratory Of Biochemistry And Molecular Biology Graduate School Of Pharmaceutical Sciences Osaka Un
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Kawai A
Laboratory Of Biochemistry And Molecular Biology Graduate School Of Pharmaceutical Sciences Osaka Un
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Kobayashi Ayako
Laboratory Of Biochemistry And Molecular Biology Graduate School Of Pharmaceutical Sciences Osaka Un
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Maeda Masatomo
Laboratory Of Biochemistry And Molecular Biology Graduate School Of Pharmaceutical Sciences Osaka Un
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