Amino Acids and Peptides. XXX. Preparation of Arg-Gly-Asp (RGD) Hybrids with Poly(Ethylene Glycol) Analogs and Their Antimetastatic Effect
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概要
- 論文の詳細を見る
Hybrids of a fibronectin-related peptide[Arg-Gly-Asp (RGD)] with poly(ethylene glycol) (PEG) analogs were prepared by a simple and easy procedure. Two amino-PEG analogs were used as carriers for hybrid formation of the RGD. One was poly(oxyethylene)dipropylamine and the other was Jeffamine ED type, which has branched chains. RGD peptides were formed stepwise on PEG analogs by the diisopropylcarbodiimide method. The synthetic intermediates were easily purified by molecular-sieve gel chromatography and the final products were purified by molecular-sieve gel chromatography, followed by HPLC. This simple and easy preparation procedure using molecular-sieve gel chromatography for purification of synthetic intermediates is advantageous for the preparation of peptide-polymer hybrids. We found that PEG is stable to HF treatment at 0℃ for 1 h. The inhibitory effect of the RGD hybrids on experimental metastasis of B16-BL6 was examined in mice. The Jeffamine type hybrid showed no inhibitory effect at the dose of 1 mg/mouse, but poly(oxyethylene)dipropylamine type hybrid was inhibitory at the same dose. The effect of the latter hybrid was about the same as that of 1 mg of RGD. One mg of the hybrid contains 0.18 μmol of RGD and 1 mg of RGD is 2.38 μmol. Thus it can be said that the inhibitory effect of RGD was potentiated by hybrid formation with poly(oxyethylene)diisopropylamine.
- 公益社団法人日本薬学会の論文
- 1997-11-15
著者
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Nakagawa S
Graduate School Of Pharmaceutical Sciences Osaka University
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Nakagawa Shinsaku
Faculty Of Pharmaceutical Sciences Osaka University
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Nakagawa Shinsaku
Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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Nakagawa Shinsaku
中華人民共和国
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Mukai Yohei
Graduate School Of Pharmaceutical Sciences Osaka University
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Mukai Yohei
医薬基盤研究所 創薬プロテオミクスプロジェクト
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Tsutsumi Y
Dep. Of Toxicology And Safety Sci. Graduate School Of Pharmaceutical Sciences Osaka Univ.
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前田 光子
Life Science Center Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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Kawai Y
Faculty Of Pharmaceutical Sciences Kobe-gakuin University
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Kawai Yuichi
Graduate School Of Pharmaceutical Sciences Kobe-gakuin Univ.
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Morishita Yuki
Dep. Of Toxicology And Safety Sci. Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Morishita Yuki
大阪大学 薬学研究科毒性学
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TSUTSUMI Yasuo
Faculty and Graduate School of Pharmaceutical Sciences, Osaka University
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MAYUMI Tadanori
Faculty and Graduate School of Pharmaceutical Sciences, Osaka University
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川崎 紘一
Life Science Center Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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Maeda M
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Nagasaki University:faculty Of Pharmac
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Maeda Mitsuko
Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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KAWASAKI Koichi
Faculty of Pharmaceutical Sciences and High Technology Research Center, Kobe Gakuin University
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KANEDA Yoshihisa
Faculty of Graduate School of Pharmaceutical Sciences, Osaka University
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Kawai Y
Safety Research Laboratory Tanabe Seiyaku Co. Ltd.
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Mayumi Tadanori
Dep. Of Biopharmaceutics Grad. Sch. Of Pharm. Sci. Osaka Univ.
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MU Yu
Faculty of Pharmaceutical Sciences, Osaka University
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IZUNO Yasuhiro
Faculty of Pharmaceutical Sciences, Kobe Gakuin University
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Mu Yu
Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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Izuno Yasuhiro
Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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Tsutsumi Yasuo
Dep. Of Biopharmaceutics Grad. Sch. Of Pharm. Sci. Osaka Univ.
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Kawasaki Keiko
Biological Science Laboratories Kao Corporation
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Kaneda Yoshihisa
Faculty Of Pharmaceutical Sciences Osaka University
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Kaneda Yoshihisa
Faculty Of Graduate School Of Pharmaceutical Sciences Osaka University
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Maeda Masatomo
Laboratory Of Biochemistry And Molecular Biology Graduate School Of Pharmaceutical Sciences Osaka Un
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Nakagawa Shinsaku
Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University
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