Production of Chimeric Mice by Exchanging Nuclei from Blastomeres of the Two-Cell Embryo
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概要
- 論文の詳細を見る
We report a new method for generating chimeras without removal of the zona pellucida. Chimeric mice were produced by exchanging nuclei from blastomeres of two-cell embryos between BALB/c and C3Hf mice. Of 108 experimental embryos, 70 (65%) survived successful exchange of nuclei between blastomeres of the two-cell embryos. Of the 70 operated embryos, 48 (69%) developed to blastocysts. Moreover, from the 154 operated two-cell embryos that were transferred to oviducts, 19 mice developed of which three were chimeras. The three chimeric mice contained both BALB/c and C3Hf mouse cells and the two types of cell were almost equally distributed. These results demonstrate that chimeras can be obtained by exchanging nuclei from blastomeres of two-cell embryos between BALB/c and C3Hf mice.
- 社団法人日本動物学会の論文
- 1993-08-16
著者
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村上 智彦
神戸学院大学 薬
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Nakagawa S
Osaka Univ. Osaka Jpn
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Nakagawa Shinsaku
Faculty Of Pharmaceutical Sciences Osaka University
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Nakagawa Shinsaku
Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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MAYUMI Tadanori
Faculty and Graduate School of Pharmaceutical Sciences, Osaka University
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HAMA Takao
Faculty of Pharmaceutical Sciences, Kobe Gakuin University
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Sumita Kousuke
Faculty of Pharmaceutical Sciences, Kobe Gakuin University
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Sumita Kousuke
Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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Nakagawa Shinsaku
Dep. Of Biotechnology And Therapeutics Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Hama T
Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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Hama Takao
Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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Hama Takao
Faculty Of Pharmaceutical Sciences Kobe-gakuin University:faculty Of Pharmaceutical Sciences Osaka U
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