Modifying the antigen-immunization schedule improves the variety of monoclonal antibodies obtained from immune-phage antibody libraries against HIV-1 Nef and Vif(MEDICAL BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
Immune phage antibody libraries are an attractive technology for isolating antigen-specific monoclonal antibodies (mAbs). Here we show that the immunization schedule affects the immune phage antibody library properties. We subcutaneously (s.c.) administered HIV-1 Nef and Vif antigens with different schedules (25μg×2s.c. and 10μg×3s.c.). The variety of isolated mAbs in 25μg×2s.c. groups (Nef: 11 clones, Vif: 9 clones) was superior to that in the 10μg×3s.c. groups (Nef: 2 clones, Vif: 1 clone). This finding suggests that it is important to optimize the immunization schedule for isolating a wide variety of mAbs.
著者
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Mukai Yohei
Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka Univ
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提 康央
Faculty Of Pharmaceutical Sciences Osaka University
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TSUNODA Shin-ichi
Laboratory of Pharmaceutical Proteomics, National Institute of Biomedical Innovation
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Tsunoda S
Lab. Of Pharmaceutical Proteomics National Inst. Of Biomedical Innovation (nibio)
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Nakagawa S
Graduate School Of Pharmaceutical Sciences Osaka University
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Nakagawa S
Osaka Univ. Osaka Jpn
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Nakagawa Shinsaku
中華人民共和国
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Mukai Yohei
Graduate School Of Pharmaceutical Sciences Osaka University
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Mukai Yohei
医薬基盤研究所 創薬プロテオミクスプロジェクト
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Sugita Toshiki
Lab. Of Pharmaceutical Proteomics National Inst. Of Biomedical Innovation
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Sugita Toshiki
医薬基盤研究所 創薬プロテオミクスプロジェクト
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Sugita Toshiki
Department Of Biopharmaceutics Graduate School Of Pharmaceutical Sciences Osaka University
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Tsutsumi Y
Dep. Of Toxicology And Safety Sci. Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Yoshioka Yasuo
Department Of Radiation Oncology Osaka University Graduate School Of Medicine
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Yoshioka Yasuo
大阪大学 医学系研究科放射線治療学
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Yoshioka Yasuo
Dept. Of Radiation Oncology Osaka University Graduate School Of Medicine
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Morishita Yuki
Dep. Of Toxicology And Safety Sci. Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Morishita Yuki
大阪大学 薬学研究科毒性学
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Tsutsumi Yasuo
Department Of Electrical Engineering Akashi College Of Technology
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Okada N
Graduate School Of Pharmaceutical Sciences Osaka University
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Yoshioka Yasuo
Dept. Of Energy Science And Engineering Musashi Inst. Of Tech.
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Tsunoda Saburo
Divisions Of Medical Oncology Tochigi Cancer Center
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Nakagawa Shinsaku
Dep. Of Biotechnology And Therapeutics Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Nakagawa Shinsaku
Department Of Biopharmaceutics Graduate School Of Pharmaceutical Sciences Osaka University
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Okada Naoki
Department Of Bioppharmaceutics Kyoto Pharmaceutical University
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堤 康央
医薬基盤研究所:大阪大学大学院薬学研究科:大阪大学臨床医工学融合研究教育センター
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Yamashiro Y
Osaka Univ. Suita Jpn
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Tsutsumi Yasuo
Dep. Of Biopharmaceutics Grad. Sch. Of Pharm. Sci. Osaka Univ.
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Yoshikawa Mai
Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka Univ
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Okada Naoki
Department Of Biopharmaceutics Kyoto Pharmaceutical University
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Nakagawa Shinsaku
Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University
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TSUTSUMI Yasuo
Department of Electrical and Computer Engineering, Akashi National College of Technology
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Yoshioka Yasuo
Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University
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