Characterization of Transcutaneous Protein Delivery by a Hydrogel Patch in Animal, Human, and Tissue-Engineered Skin Models
スポンサーリンク
概要
- 論文の詳細を見る
The development of a simple, easy-to-use, and noninvasive vaccination system is in high demand. For transcutaneous immunization (TCI), we previously reported that a hydrogel patch was an effective TCI device that accelerates antigen penetration through the stratum corneum in mouse and rat models. The present study was performed to characterize the transcutaneous protein delivery induced by the hydrogel patch in mouse, guinea pig, LWD pig, human, or tissue-engineered skin models, and to assess the activity of proteins delivered into the skin. The hydrogel patch promoted protein penetration through the stratum corneum in all skin models, indicating that our original hydrogel patch might have practical application for use in humans. In addition, proteins delivered into the skin by the hydrogel patch retained their activity, suggesting that the hydrogel patch is applicable for the delivery of therapies for other diseases as well. On the basis of these results, translational research in human is now in progress.
著者
-
Mukai Yohei
Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka Univ
-
Nakagawa Shinsaku
Department Of Biopharmaceutics Graduate School Of Pharmaceutical Sciences Osaka University
-
Okada Naoki
Department Of Bioppharmaceutics Kyoto Pharmaceutical University
-
Kamiyama Fumio
Cosmed Pharmaceutical Co. Ltd.
-
Ishii Yumiko
Department Of Chemical Pharmacology Toho University School Of Pharmaceutical Sciences
-
Matsuo Kazuhiko
Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka Univ
-
Quan Ying-Shu
CosMED Pharmaceuticals Co., Ltd.
-
Okada Naoki
Department Of Biopharmaceutics Kyoto Pharmaceutical University
関連論文
- Optimized PEGylated Adenovirus Vector Reduces the Anti-vector Humoral Immune Response against Adenovirus and Induces a Therapeutic Effect against Metastatic Lung Cancer
- In Vitro and In Vivo Tumor Suppressive Activity Induced by Human Telomerase Transcriptase-Targeting Antisense Oligonucleotides Mediated by Cationic Liposomes(CELL AND TISSUE ENGINEERING)
- Cell-Penetrating Peptides as Adenovirus Vector Carrier
- Creation of Novel Cell-Penetrating Peptides for Intracellular Drug Delivery Using Systematic Phage Display Technology Originated from Tat Transduction Domain(Biochemistry)
- Non-Methylated CpG Motif Packaged into Fusogenic Liposomes Enhance Antigen-Specific Immunity in Mice(Highlighted paper selected by Editor-in-chief,Pharmacology)
- Vaccine Efficacy of Fusogenic Liposomes Containing Tumor Cell-Lysate against Murine B16BL6 Melanoma(Pharmacology)
- Anti-tumor Responses Induced by Chemokine CCL19 Transfected into an Ovarian Carcinoma Model via Fiber-Mutant Adenovirus Vector(Biopharmacy)
- Fusogenic Liposome can be Used as an Effective Vaccine Carrier for Peptide Vaccination to Induce Cytotoxic T Lymphocyte (CTL) Response(Biopharmacy)
- Creation of Novel Protein Transduction Domain (PTD) Mutants by a Phage Display-Based High-Throughput Screening System(Biochemistry)
- Quality Enhancement of the Non-immune Phage scFv Library to Isolate Effective Antibodies(Highlighted paper selected by Editor-in-chief,Biochemistry)
- Selective Enhancer of Tumor Vascular Permeability for Optimization of Cancer Chemotherapy (Pharmacognosy)
- Effective Cancer Targeting Using an Anti-tumor Tissue Vascular Endotheliumm specific Monoclonal Antibody (TES-23)
- Prolongation of the Effective Duration of Cytomedical Therapy by Re-injecting SK2 Hybridoma Cells Microencapsulated within Alginate-Poly(L)lysine-Alginate Membranes into Human Interleukin-6 Transgenic Mice
- Anti-angiogenic Effects of Dimethyl Sulfoxide on Endothelial Cells(Pharmacognosy)
- Preparation and Biological Activities of a Bivalent Poly(Ethylene Glycol) Hybrid Containing an Active Site and Its Synergistic Site of Fibronectin
- Tumor Necrosis Factor α-Gene Therapy for an Established Murine Melanoma Using RGD (Arg-Gly-Asp) Fiber-mutant Adenovirus Vectors
- Cell Delivery System : A Novel Strategy to Improve the Efficacy of Cancer Immunotherapy by Manipulation of Immune Cell Trafficking and Biodistribution
- Effects of Labrasol and Other Pharmaceutical Excipients on the Intestinal Transport and Absorption of Rhodamine123, a P-Glycoprotein Substrate, in Rats(Biopharmacy)
- 難吸収性薬物の消化管吸収に対するNO供与体の吸収促進効果の特性 : 従来型の吸収促進剤との比較
- Evaluation of Insulin Permeability and Effects of Absorption Enhancers on Its Permeability by an in Vitro Pulmonary Epithelial System Using Xenopus Pulmonary Membrane
- Preferential Activity of Wild-type and Mutant Tumor Necrosis Factor-α against Tumor-derived Endothelial-like Cells
- Transdermal Delivery of Insulin Using Trypsin as a Biochemical Enhancer(Biopharmacy)
- The Utility of Poly(γ-glutamic acid) Nanoparticles as Antigen Delivery Carriers in Dendritic Cell-Based Cancer Immunotherapy
- A Phagocytotic Inducer from Herbal Constituent, Pentagalloylglucose Enhances Lipoplex-Mediated Gene Transfection in Dendritic Cells
- Characterization of Transcutaneous Protein Delivery by a Hydrogel Patch in Animal, Human, and Tissue-Engineered Skin Models
- Enhanced Permeability of Phenylalanyl-glycine (Phe-Gly) Across the Intestinal Membranes by Chemical Modification with Various Fatty Acids
- Rapid adaptation : a new dimension for evolutionary perspectives in ecology
- Modifying the antigen-immunization schedule improves the variety of monoclonal antibodies obtained from immune-phage antibody libraries against HIV-1 Nef and Vif(MEDICAL BIOTECHNOLOGY)
- Development of Novel Technology of DDS for Gene Therapy
- Competitive exclusion between contest and scramble strategists in Callosobruchus seed-beetle modeling
- SPECIFIC 3H-HALOPERIDOL BINDING TO DOPAMINE RECEPTORS IN THE ANTERIOR BYSSUS RETRACTOR MUSCLE OF MYTILUS EDULIS
- EFFECTS OF SOME ERGOT ALKALOIDS ON DOPAMINE RECEPTORS OF MOLLUSCAN SMOOTH MUSCLE
- The effect of learning and search images on predator-prey interactions
- PHARMACOLOGICAL PROPERTIES OF ACETYLCHOLINE RECEPTOR IN MOLLUSCAN SMOOTH MUSCLE
- Modifying the antigen-immunization schedule improves the variety of monoclonal antibodies obtained from immune-phage antibody libraries against HIV-1 Nef and Vif
- Pseudo-Meigs’Syndrome as a Cause of Exertional Dyspnea: A Case Report
- Comparison of Leukotriene Receptor Antagonists and Anti-Histamines as an Add-On Therapy in Patients with Asthma Complicated by Allergic Rhinitis
- Intravenous Administration of Polyethylene Glycol-Coated (PEGylated) Proteins and PEGylated Adenovirus Elicits an Anti-PEG Immunoglobulin M Response
- Compositional Optimization and Safety Assessment of a Hydrogel Patch as a Transcutaneous Immunization Device
- In Vitro and In Vivo Tumor Suppressive Activity Induced by Human Telomerase Transcriptase-Targeting Antisense Oligonucleotides Mediated by Cationic Liposomes
- Concurrent Lung Cancer in Non-Tuberculous Mycobacteriosis: Case Report