A Phagocytotic Inducer from Herbal Constituent, Pentagalloylglucose Enhances Lipoplex-Mediated Gene Transfection in Dendritic Cells

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概要

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• Antigen-presenting cells are key vehicles for delivering antigens in tumor immunotherapy, and the most potent of them are dendritic cells (DCs). Recent studies have demonstrated the usefulness of DCs genetically modified by lipofection in tumor immune therapy, although sufficient gene transduction into DCs is quite difficult. Here, we show that Paeoniae radix, herbal medicine, and the constituent, 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG), have an attractive function to enhance phagocytosis in murine dendritic cell lines, DC2.4 cells. In particular, PGG in combination with lipofectin (LPF) enhanced phagocytic activity. Furthermore, PGG enhanced lipofection efficacy in DC2.4 cells, but not in colorectal carcinoma cell lines, Colon26. In other words, PGG synergistically enhanced the effect of lipofectin-dependent phagocytosis on phagocytic cells. Hence, according to our data, PGG could be an effective aid in lipofection using dendritic cells. Furthermore, these findings provide an expectation that constituents from herbal plant enhance lipofection efficacy.

著者

• Nakanishi Tsuyoshi

  Laboratory of Hygienics, Gifu Pharmaceutical University

• Nakagawa Shinsaku

  Department Of Biopharmaceutics Graduate School Of Pharmaceutical Sciences Osaka University

• Saiki Ikuo

  Division Of Pathogenic Biochemistry Department Of Bioscience Institute Of Natural Medicine Universit

• Nakanishi Tsuyoshi

  Laboratory Of Hygienics Gifu Pharmaceutical University:department Of Toxicology Graduate School Of P

• Sakurai Hiroaki

  Division Of Pathogenic Biochemistry Institute Of Natural Medicine University Of Toyama

• Koizumi Keiichi

  Division Of Pathogenic Biochemistry Department Of Bioscience Institute Of Natural Medicine Universit

• Yamada Miyuki

  Division Of Linguistics And Literature Graduate School Of Letters Hokkaido University

• Kato Shinichiro

  Division of Pathogenic Biochemistry, Institute of Natural Medicine, University of Toyama

• Inujima Akiko

  Division of Pathogenic Biochemistry, Institute of Natural Medicine, University of Toyama

• Takeno Nobuhiro

  Division of Pathogenic Biochemistry, Institute of Natural Medicine, University of Toyama

• Nakanishi Tsuyoshi

  Laboratory Of Hygienic Chemistry And Molecular Toxicology Gifu Pharmaceutical University

• Yamada Miyuki

  Division of Pathogenic Biochemistry, Institute of Natural Medicine, University of Toyama

• Koizumi Keiichi

  Division of Pathogenic Biochemistry, Institute of Natural Medicine, University of Toyama

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