Adenovirus Vector Covalently Conjugated to Polyethylene Glycol with a Cancer-Specific Promoter Suppresses the Tumor Growth through Systemic Administration
スポンサーリンク
概要
- 論文の詳細を見る
Cancer gene therapy with adenovirus vectors (Adv) is limited to local administration because systemic administration of Adv produces a weak therapeutic effect and severe side effects. Previously, we generated a dual cancer-specific Adv system by using Adv covalently conjugated to polyethylene glycol (PEG) for transductional targeting and the telomere reverse transcriptase (TERT) promoter as a cancer-specific promoter for transcriptional targeting (PEG-Ad-TERT). We demonstrated that systemic administration of PEG-Ad-TERT showed superior antitumor effects against lung metastatic cancer with negligible side effects. Here, we investigated the therapeutic efficacy of systemic administration of PEG-Ad-TERT for the treatment of primary tumors. We first evaluated the transgene expression of PEG-Ad-TERT containing the luciferase gene (PEG-Ad-TERT/Luc) in primary tumors. Systemic administration of PEG-Ad-TERT/Luc resulted high transgene expression, similar to that observed in tumors for the conventional cytomegalovirus (CMV) promoter-driven Adv containing the luciferase gene (Ad-CMV/Luc). By comparison, transgene expression was 2500-fold lower than that of Ad-CMV/Luc in liver. We then examined the therapeutic effect of systemic administration of PEG-Ad-TERT containing the herpes simplex virus thymidine kinase (HSVtk) gene (PEG-Ad-TERT/HSVtk) for the treatment of primary tumors. We showed that PEG-Ad-TERT/HSVtk produced a notable antitumor effect against primary tumors with negligible side effects. These results demonstrated that PEG-Ad-TERT can be regarded as a prototype Adv with suitable efficacy and safety for systemic cancer gene therapy against both metastatic and primary tumors.
- 日本薬学会の論文
著者
-
Mizuguchi Hiroyuki
Department of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka
-
MUKAI Yohei
Laboratory of Pharmaceutical Proteomics, National Institute of Biomedical Innovation
-
YOSHIOKA Yasuo
Laboratory of Pharmaceutical Proteomics, National Institute of Biomedical Innovation (NiBio)
-
Yoshioka Yasuo
Department Of Radiation Oncology Osaka University Graduate School Of Medicine
-
Yoshioka Yasuo
大阪大学 医学系研究科放射線治療学
-
Yoshioka Yasuo
Dept. Of Radiation Oncology Osaka University Graduate School Of Medicine
-
Yao Xinglei
Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka Univ
-
Morishige Tomohiro
Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka Univ
-
Eto Yusuke
Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka Univ
-
Narimatsu Shogo
Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka Univ
-
Okada Naoki
Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka Univ
-
Nakagawa Shinsaku
Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka Univ
-
Yamashiro Y
Osaka Univ. Suita Jpn
-
Yoshioka Yasuo
Laboratory Of Biopharamceutical Research National Institute Of Biomedical Innovation
-
Narimatsu Shogo
Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University
-
Nakagawa Shinsaku
Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University
-
Mukai Yohei
Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University
-
Yao Xinglei
Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University
-
Yoshioka Yasuo
Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University
-
Okada Naoki
Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University
-
Eto Yusuke
Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University
関連論文
- Urban Aerosols Induce Pro-inflammatory Cytokine Production in Macrophages and Cause Airway Inflammation in Vivo
- Optimized PEGylated Adenovirus Vector Reduces the Anti-vector Humoral Immune Response against Adenovirus and Induces a Therapeutic Effect against Metastatic Lung Cancer
- Cell-Penetrating Peptides as Adenovirus Vector Carrier
- Creation of Novel Cell-Penetrating Peptides for Intracellular Drug Delivery Using Systematic Phage Display Technology Originated from Tat Transduction Domain(Biochemistry)
- Anti-tumor Responses Induced by Chemokine CCL19 Transfected into an Ovarian Carcinoma Model via Fiber-Mutant Adenovirus Vector(Biopharmacy)
- Fast Binding Kinetics and Conserved 3D Structure Underlie the Antagonistic Activity of Mutant TNF : Useful Information for Designing Artificial Proteo-Antagonists
- Creation of Novel Protein Transduction Domain (PTD) Mutants by a Phage Display-Based High-Throughput Screening System(Biochemistry)
- Quality Enhancement of the Non-immune Phage scFv Library to Isolate Effective Antibodies(Highlighted paper selected by Editor-in-chief,Biochemistry)
- Identification of epigallocatechin-3-O-gallate as an active constituent in tea extract that suppresses transcriptional up-regulations of the histamine H_1 receptor and interleukin-4 genes
- What is the Optimum Minimum Segment Size Used in Step and Shoot IMRT for Prostate Cancer?
- Selective Enhancer of Tumor Vascular Permeability for Optimization of Cancer Chemotherapy (Pharmacognosy)
- High-dose-rate Brachytherapy Combined with Long-term Hormonal Therapy for High-risk Prostate Cancer : Results of a Retrospective Analysis
- Anti-angiogenic Effects of Dimethyl Sulfoxide on Endothelial Cells(Pharmacognosy)
- Preparation and Biological Activities of a Bivalent Poly(Ethylene Glycol) Hybrid Containing an Active Site and Its Synergistic Site of Fibronectin
- 3P-081 ファージ表面提示法を用いたTNFR1指向性リンフォトキシンαの創製とその特性評価(蛋白質工学,進化工学,第46回日本生物物理学会年会)
- Selective Enhancement by Tumor Necrosis Factor-α of Vascular Permeability of New Blood Vessels Induced with Agarose Hydrogel-entrapped Meth-A Fibrosarcoma Cells
- Novel correction methods as alternatives for the six-dimensional correction in CyberKnife treatment
- Late complications after high-dose-rate interstitial brachytherapy for tongue cancer
- Brachytherapy for Early Oral Tongue Cancer : Low Dose Rate to High Dose Rate
- Changes in Performance Status of Elderly Patients after Radiotherapy
- Role of Mitochondrial DNA in Radiation Exposure
- Ultrasonographic Monitoring of High Dose Rate Interstitial Implant using Template Technique for Oral Tongue Cancer
- OS4(5)-20(OS04W0320) Residual Stress Analyses in Laser Single Pulse Irradiated Area Using Synchrotron Radiation
- X-Ray Fractography Using Synchrotron Radiation : Residual Stress Distribution Just beneath Fatigue Fracture Surface
- New ambulatory implant technique of high-dose-rate interstitial brachytherapy for prostate cancer
- Kujin Suppresses Histamine Signaling at the Transcriptional Level in Toluene 2,4-Diisocyanate-Sensitized Rats
- Repeated Pre-treatment With Antihistamines Causes Transcriptional Up-regulations of Histamine H_1 Receptor and Interleukin-4 Genes in Toluene-2,4-diisocyanate-Sensitized Rats
- Evaluation of Efficacy and Sedative Profiles of H_1 Antihistamines by Large-Scale Surveillance Using the Visual Analogue Scale (VAS)
- Amino Acids and Peptides.XXXVIII.Facile Synthesis of Laminin-Related Peptide-Poly(Ethylene Glycol)Hybrids by the Solid Phase Method
- Arsenic Trioxide Inhibits Human T Cell-Lymphotropic Virus-1-Induced Syncytiums by Down-Regulating gp46
- Sho-seiryu-to Suppresses Histamine Signaling at the Transcriptional Level in TDI-Sensitized Nasal Allergy Model Rats
- Suppression of Histamine Signaling by Probiotic Lac-B : a Possible Mechanism of Its Anti-allergic Effect
- A Bivalent Poly (ethylene glycol) Hybrid Containing an Active Site (RGD) and a Synergistic Site of Fibronectin
- In vivo Anti-tumor Efficacy of Polyethylene Glycol-modified Tumor Necrosis Factor-α against Tumor Necrosis Factor-resistant Tumors
- Purification and Characterization of [^3H] Mepyramine (Histamine H_1 Antagonist)-Binding Protein from Rat Liver: A Highly Homologous Protein with Cytochrome P450 2D
- Stimulation of Histamine H_1 Receptor Up-Regulates Histamine H_1 Receptor Itself Through Activation of Receptor Gene Transcription
- Preparation of Laminin γ1 Chain Related Peptide Poly (Ethylene Glycol) Hybrid
- Amino Acids and Peptides. XXIX. Synthesis and Antimetastatic Effects of Peptides and Peptide-Poly(Ethylene Glycol) Hybrids Related to the Core Sequence of the Type III Connecting Segment Domain of Fibronectin
- The Utility of Poly(γ-glutamic acid) Nanoparticles as Antigen Delivery Carriers in Dendritic Cell-Based Cancer Immunotherapy
- Adenovirus Vector Covalently Conjugated to Polyethylene Glycol with a Cancer-Specific Promoter Suppresses the Tumor Growth through Systemic Administration
- Effect of KAFDITYVRLK(C-16), a Laminin Gamma 1 Chain Peptide, to Tumor Cell Adhesion and Metastasis
- Identification of Biologically Active Peptides in Laminin-1
- Current status and perspectives of brachytherapy for prostate cancer
- Verification of air-kerma strength of ^I seed for permanent prostate implants in Japan
- Structure of Imidazole Glycerol Phosphate Synthase from Thermus thermophilus HB8 : Open-Closed Conformational Change and Ammonia Tunneling
- Glutamine : phenylpyruvate Aminotransferase from an Extremely Thermophilic Bacterium, Thermus thermophilus HB8
- Functionally Important Residues of Aromatic L-Amino Acid Decarboxylase Probed by Sequence Alignment and Site-Directed Mutagenesis
- Modifying the antigen-immunization schedule improves the variety of monoclonal antibodies obtained from immune-phage antibody libraries against HIV-1 Nef and Vif(MEDICAL BIOTECHNOLOGY)
- Preliminary Results of Magnetic Resonance Imaging-aided High-dose-rate Interstitial Brachytherapy for Recurrent Uterine Carcinoma after Curative Surgery
- Safety Evaluation Study of Nanomaterials Aimed at Promoting Their Acceptance by Society
- Modifying the antigen-immunization schedule improves the variety of monoclonal antibodies obtained from immune-phage antibody libraries against HIV-1 Nef and Vif
- Inverse Agonistic Activity of Antihistamines and Suppression of Histamine H_1 Receptor Gene Expression
- Intravenous Administration of Polyethylene Glycol-Coated (PEGylated) Proteins and PEGylated Adenovirus Elicits an Anti-PEG Immunoglobulin M Response
- Compositional Optimization and Safety Assessment of a Hydrogel Patch as a Transcutaneous Immunization Device
- Combination of Leukotoriene Receptor Antagonist With Antihistamine Has an Additive Suppressive Effect on the Up-regulation of H1-Receptor mRNA in the Nasal Mucosa of Toluene 2,4-Diisocyanate-Sensitized Rat
- S1-3 Clinical significance of antihistamines(Antihistamine update (English Session))
- Identification of epigallocatechin-3-O-gallate as an active constituent in tea extract that suppresses transcriptional up-reguiations of the histamine H_1 receptor and interleukin-4 genes