A Baculoviral Display System to Assay Viral Entry

スポンサーリンク

概要

• 論文の詳細を見る

• In this study, we evaluated a baculoviral display system for analysis of viral entry by using a recombinant adenovirus (Ad) carrying a luciferase gene and budded baculovirus (BV) that displays the adenoviral receptor, coxsackievirus and adenovirus receptor (CAR). CAR-expressing B16 cells (B16-CAR cells) were infected with luciferase-expressing Ad vector in the presence of BV that expressed or lacked CAR (CAR-BV and mock-BV, respectively). Treatment with mock-BV even at doses as high as 5 µg/mL failed to attenuate the luciferase activity of B16-CAR cells. In contrast, treatment with CAR-BV with doses as low as 0.5 µg/mL significantly decreased the luciferase activity of infected cells, which reached 65% reduction at 5 µg/mL. These findings suggest that a receptor-displaying BV system could be used to evaluate viral infection.

• 公益社団法人 日本薬学会の論文

著者

• KONDOH Masuo

  Laboratory of Antibiotics, The Institute of Physical and Chemical Research (RIKEN)

• Yagi Kiyohito

  Laboratory Of Bio-functional Engineering Faculty Of Pharmaceutical Sciences Osaka University

• Hamakubo Takao

  Department Of Molecular Biology And Medicine Rcast # 35 University Of Tokyo

• Yoshida Takeshi

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Iida Manami

  Laboratory of Bio-Functional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University

• Watari Akihiro

  Laboratory of Bio-Functional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University

• Yoshida Takeshi

  Laboratory of Bio-Functional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University

• Kondoh Masuo

  Laboratory of Bio-Functional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University

関連論文

• Downregulated P1 promoter-driven hepatocyte nuclear factor-4α expression in human colorectal carcinoma is a new prognostic factor against liver metastasis
• The Effect of Statins on mRNA Levels of Genes Related to Inflammation, Coagulation, and Vascular Constriction in HUVEC
• Transcriptional Activation by Hypoxia and Low-Density Lipoprotein Loading in Cultured Vascular Smooth Muscle Cells
• Analysis of the Global RNA Expression Profiles of Skeletal Muscle Cells Treated with Statins
• Global Analysis of RNA Expression Profile in Human Vascular Cells Treated with Statins
• Extensive Oligonucleotide Microarray Transcriptome Analysis of the Rat Cerebral and Arachnoid Tissue
• Gene Expression Induced by BO-653, Probucol and BHQ in Human Endothelial Cells
• Relative Induction of mRNA for HMG CoA Reductase and LDL Receptor by Five Different HMG-CoA Reductase Inhibitors in Cultured Human Cells
• A Comparison of Differences in the Gene Expression Profiles of Phorbol 12-myristate 13-acetate Differentiated THP-1 Cells and Human Monocyte-derived Macrophage
• Genomic Structure and Mapping of Human Orphan Receptor LXR Alpha : Upregulation of LXRa mRNA During Monocyte to Macrophage Differentiation
• The Gene Expression Profile of Human Umbilical Vein Endothelial Cells Stimulated by Tumor Necrosis Factor α Using DNA Microarray Analysis
• Separate elements are required for ligand-dependent and -independent internalization of metastatic potentiator CXCR4
• Effects of Tryprostatin Derivatives on Microtubule Assembly In Vitro and In Situ
• A Novel Zinc Finger Protein mRNA in Human Umbilical Vein Endothelial Cells is Profoundly Induced by Tumor Necrosis Factor α
• ブタ水晶体グルタチオンS-トランスフェラーゼの光化学的失活に及ぼすフルクトースの影響
• Modulation of Subcellular Particles of the Rat Small Intestine and Liver by Ebselen
• Measurement of HCV RdRp activity with C-terminal 21 aa truncated NS5b protein : optimization of assay conditions
• Genomic structure of the gene encoding human 3-hydroxy-3-methyl-glutaryl coenzyme A reductase : comparison of exon/intron organization of sterol-sensing domains among four related genes
• Genomic structure and chromosomal mapping of the human sterol regulatory element binding protein (SREBP) cleavage-activating protein (SCAP) gene
• The Generation of Monoclonal Antibodies against Human Peroxisome Proliferator-activated Receptors(PPARs)
• Increase in Thioredoxin Activity of Intestinal Epihelial Cells Mediated by Oxidative Stress
• Human Calpain 7/PalBH Associates with a Subset of ESCRT-III-related Proteins in its N-terminal Region and Partly Localizes to Endocytic Membrane Compartments
• Immunohistochemical staining for P1 and P2 promoter-driven hepatocyte nuclear factor-4α may complement mucin phenotype of differentiated-type early gastric carcinoma
• Expression of liver X receptor α and lipid metabolism in granulocyte-macrophage colony-stimulating factor-induced human monocyte-derived macrophage
• Protein expression of nuclear receptors in human and murine tissues
• Expression of hepatocyte nuclear factor 4α in primary ovarian mucinous tumors
• Missense Mutation in Abcg5 in SHRSP Rats Does Not Accelerate Intestinal Absorption of Plant Sterols : Comparison with Wistar Rats
• N-linked glycan-dependent interaction of CD63 with CXCR4 at the Golgi apparatus induces downregulation of CXCR4
• Identification of Nucleotide Residues Essential for RNase P Activity from the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3
• Recent Advances in Claudin-Targeting Technology
• A Baculoviral Display System to Assay Viral Entry

もっと見る 閉じる

スポンサーリンク