Two-dimensional electrophoretic analysis of disease-associated proteins in human cerebrospinal fluid from patients with rheumatoid arthritis

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概要

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• Comparing protein expression in the cerebrospinal fluid (CSF) of rheumatoid arthritis (RA) patients with that of controls, makes possible the uncovering of proteins that affect disease progression and regulate responsiveness to drugs. Two-dimensional gel electrophoresis (2-DE) and silver staining were used for identifying disease-associated CSF proteins in RA patients. First, to enhance the detection of CSF proteins and to improve the separation of their isoforms by 2-DE, CSF samples were pre-treated with an albumin and IgG removal kit, then by acetone precipitation. The 2-DE analysis revealed more than 1600 spots by the removal of albumin and immunoglobulin from CSF. The expression of the protein spots was not greatly changed in either group, but some notable changes in protein spots were observed in two RA samples. In particular, the expression of an approximately 50 kD protein increased markedly, whereas that of two sequential protein spots of 10-15 kD and with neutral pI decreased in the RA samples. These preliminary results suggest that the proteomic method is conducive to clarifying the mechanism of RA crises, and that some of the expression-changed proteins may be new candidates for disease-associated proteins of RA.

• 2005-03-01

著者

• Yamaguchi T

  Division Of Cellular And Gene Therapy Products National Institute Of Health Sciences

• Yamamoto Y

  Department Of Biological Production Faculty Of Bioresource Sciences Akita Prefectural University

• Hayakawa T

  Pharmaceutical Res. And Technol. Inst. Kinki Univ.

• Hayakawa T

  National Institute Of Health Sciences

• Yamaguchi Teruhide

  National Institute Of Health Science

• YAMAMOTO Yukio

  Tokyo Metropolitan Institute of Medical Science

• AKITA Yoshiko

  Tokyo Metropolitan Institute of Medical Science

• TAI Shigeyuki

  Tokyo Metropolitan Bokutoh Hospital

• FUKASAKU Susumu

  School of Medicine, Juntendo University

• OSHIZAWA Tadashi

  National Institute of Health Science

• YAMAOKA Kazuko

  Tokyo Metropolitan Institute of Medical Science

• SHIMAMURA Mariko

  Tokyo Metropolitan Institute of Medical Science

• HAZATO Tadahiko

  Tokyo Metropolitan Institute of Medical Science

• OSHIZAWA Tadashi

  Division of Cellular and Gene Therapy Products National Institute of Health Sciences

• Shimamura Mariko

  Department Of Medical Biology Tokyo Metropolitan Institute Of Medical Science

• Yamamoto Y

  Faculty Of Agriculture Kochi University

• Yamamoto Yoshinori

  Kochi Univ. Kochi Jpn

• FUKASAKU Susumu

  Department of Orthopaedic Surgery, School of Medicine, Juntendo University

• TAI Sigeyuki

  Department of Anesthesiology, Tokyo Metropolitan Bokutoh Hospital

• HAZATO Tadahiko

  Department of Medical Biology, Tokyo Metropolitan Institute of Medical Science

• Tai Sigeyuki

  Department Of Anesthesiology Tokyo Metropolitan Bokutoh Hospital

• Fukasaku Susumu

  Department Of Orthopaedic Surgery School Of Medicine Juntendo University

• Hazato Tadahiko

  Department Of Medical Biology Tokyo Metropolitan Institute Of Medical Science

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