Characterization of an Anion Transporter in the Plasma Membrane of Barley Roots
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概要
- 論文の詳細を見る
To examine the relationship between H^+-ATPase and the transport of anions, we investigated the effects of various inhibitors on the activity of the H^+-ATPase, the transport of protons, and the transport of Cl^- ions using plasma membrane vesicles prepared from barley roots. Some inhibitors, namely, 4,4-diisothiocyano-2,2-stilbene disulfonate (DIDS) and Zn^<2+> ions markedly inhibited H^+- ATPase activity. Other compounds, such as phenylglyoxal (PGO) and niflumic acid (NIF), inhibited H^+-ATPase activity by 20-30%, while anthracene-9-carboxylate (A-9-C) and tetraethylammonium chloride (TEA-Cl) had little effect on this activity. The ATP-dependent acidification of the interior of vesicles was strongly dependent on the presence of permeant anions, such as chloride (Cl^-) and nitrate (NO^-_3), and it was completely inhibited by 0.2 mM NIF. Other compounds, namely, A-9-C of 0.1 mM and TEA-CI of 10 mM, did not affect H^+-transport activity. The inhibition of H^+-transport activity by NIF was observed even when the activity was assayed in the presence of KCl, KNO_3, or bis-tris-propane (BTP)-Cl. Using ^<36>Cl^-, we quantified Cl^-transport activity by measuring the uptake of Cl^- ions into the plasma membrane vesicles. The uptake depended on the potential difference across the membrane that was generated by H^+-ATPase; it was enhanced by an inside-positive potential gradient. At 0.1 mM, NIF completely blocked the voltage-dependent Cl^-transport activity. From these properties of the Cl^- transporter and the inhibition of H^+- transport activity by NIF, we suggest that H^+-transport activity across the plasma membrane might be modulated by the transport of anions via a NIF-sensitive anion-permeable transporter that acts to collapse the inside-positive potential generated by H^+-ATPase.
- 日本植物生理学会の論文
著者
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Yamamoto Yoko
Research Institute For Bioresources Okayama University
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Matsumoto Hideaki
Res. Inst. Bioresources Okayama Univ.
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Matsumoto Hideaki
Research Institute For Bioresources Okayama University
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Yamamoto Y
Faculty Of Agriculture Kochi University
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Yamamoto Yoshinori
Kochi Univ. Kochi Jpn
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Yamashita Kousei
Research Institute For Bioresources Okayama University
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Matsumoto Hideaki
Res. Inst. For Bioresources Okayama Univ.
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