Genes involved in novel adaptive aluminum resistance in Rhodotorula glutinis(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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概要

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• Rhodotorula glutinis IFO1125 acquired increased aluminum (Al) resistance from 50μM to more than 5mM by repetitive culturing with stepwise increases in Al concentration at pH4.0. In our previous study we performed differential display to find that three genes (RgFET3, RgGET3, and RgCMK) encoding proteins homologous to Saccharomyces cerevisiae FET3p, GET3p, and CMK1p and CMK2p, respectively, were up-regulated in the Al-resistant cells. In this study we cloned these genes and found they were indeed up-regulated in Al-resistant strains. The cloned genes were introduced into S. cerevisiae and corresponding mutants to test their relevance to Al resistance. The introduction of RgFET3 and RgGET3 conferred Al resistance to the host, but that of RgCMK did not. Green fluorescent protein (GFP)-tagged RgFet3p was localized at the cell periphery in the host. GFP-tagged RgGet3p formed more punctate bodies in the host under Al stress than in the absence of Al. Different growth responses to Fe (III), Cu (II), Ca ions, and cyclosporin A in the wild type and resistant cells of R. glutinis suggested the involvement and possible links of the three genes in Al resistance.

著者

• Tani Akio

  Research Institute for Bioresources, Okayama University

• Kawahara Takaya

  Research Institute for Bioresources, Okayama University

• Yamamoto Yoko

  Research Institute for Bioresources, Okayama University

• Kimbara Kazuhide

  Research Institute for Bioresources, Okayama University

• Kawai Fusako

  Research Institute for Bioresources, Okayama University

• Yamamoto Yoko

  Research Institute For Bioresources Okayama University

• Kawahara Takaya

  Research Institute For Bioresources Okayama University

• Kimbara Kazuhide

  Research Institute For Bioresources Okayama University

• Kimbara Kazuhide

  Research Institute For Bioresouces Okayama University

• Kimbara Kazuhide

  Res. Inst. Biores. Okayama Univ.

• Tani Akio

  Research Institute For Bioresouces Okayama University

• Kawai Fusako

  Research Institute For Bioresources Okayama University:(present Office)r & D Center For Bio-base

• Kawai Fusako

  Research Institute For Bioresources Okayama University

• TANI Akio

  Institute of Plant Science and Resources, Okayama University

• Kawai Fusako

  Res. Inst. Biores. Okayama Univ.

• Tani Akio

  Institute Of Plant Science And Resources Okayama University

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