Genes involved in novel adaptive aluminum resistance in Rhodotorula glutinis(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
Rhodotorula glutinis IFO1125 acquired increased aluminum (Al) resistance from 50μM to more than 5mM by repetitive culturing with stepwise increases in Al concentration at pH4.0. In our previous study we performed differential display to find that three genes (RgFET3, RgGET3, and RgCMK) encoding proteins homologous to Saccharomyces cerevisiae FET3p, GET3p, and CMK1p and CMK2p, respectively, were up-regulated in the Al-resistant cells. In this study we cloned these genes and found they were indeed up-regulated in Al-resistant strains. The cloned genes were introduced into S. cerevisiae and corresponding mutants to test their relevance to Al resistance. The introduction of RgFET3 and RgGET3 conferred Al resistance to the host, but that of RgCMK did not. Green fluorescent protein (GFP)-tagged RgFet3p was localized at the cell periphery in the host. GFP-tagged RgGet3p formed more punctate bodies in the host under Al stress than in the absence of Al. Different growth responses to Fe (III), Cu (II), Ca ions, and cyclosporin A in the wild type and resistant cells of R. glutinis suggested the involvement and possible links of the three genes in Al resistance.
著者
-
Tani Akio
Research Institute for Bioresources, Okayama University
-
Kawahara Takaya
Research Institute for Bioresources, Okayama University
-
Yamamoto Yoko
Research Institute for Bioresources, Okayama University
-
Kimbara Kazuhide
Research Institute for Bioresources, Okayama University
-
Kawai Fusako
Research Institute for Bioresources, Okayama University
-
Yamamoto Yoko
Research Institute For Bioresources Okayama University
-
Kawahara Takaya
Research Institute For Bioresources Okayama University
-
Kimbara Kazuhide
Research Institute For Bioresources Okayama University
-
Kimbara Kazuhide
Research Institute For Bioresouces Okayama University
-
Kimbara Kazuhide
Res. Inst. Biores. Okayama Univ.
-
Tani Akio
Research Institute For Bioresouces Okayama University
-
Kawai Fusako
Research Institute For Bioresources Okayama University:(present Office)r & D Center For Bio-base
-
Kawai Fusako
Research Institute For Bioresources Okayama University
-
TANI Akio
Institute of Plant Science and Resources, Okayama University
-
Kawai Fusako
Res. Inst. Biores. Okayama Univ.
-
Tani Akio
Institute Of Plant Science And Resources Okayama University
関連論文
- Flow cytometry-based method for isolating live bacteria with meta-cleavage activity on dihydroxy compounds of biphenyl(METHODS)
- Genes involved in novel adaptive aluminum resistance in Rhodotorula glutinis(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Cloning and Expression of Soluble Cytochrome c and Its Role in Polyvinyl Alcohol Degradation by Polyvinyl Alcohol-Utilizing Sphingopyxis sp. Strain 113P3(ENVIRONMENTAL BIOTECHNOLOGY)
- Characterization of a Cryptic Plasmid, pSM103mini, from Polyethylene-Glycol Degrading Sphingopyxis macrogoltabida Strain 103
- Two Acyl-CoA Dehydrogenases of Acinetobacter sp. Strain M-1 That Uses Very Long-Chain n-Alkanes
- A Non-Conventional Dissimilation Pathway for Long Chain n-Alkanes in Acinetobacter sp. M-1 That Starts with a Dioxygenase Reaction
- Sequence Upstream of the Wheat (Triticum aestivum L.) ALMT1 Gene and its Relationship to Aluminum Resistance
- Evidence for the Plasma Membrane Localization of Al-activated Malate Transporter (ALMT1)
- Enhancement of H^+-ATPase activity in purple lupin (Lupinus hirsutus) in response to phosphorus deficiency and its role in the exudation of organic anions
- ALUMINUM INDUCED ALTERATION IN THE INTRACELLULAR [Ca^] AND MICROTUBULES IN TOBACCO CELLS
- Phenylpropanoids as a Protectant of Aluminum Toxicity in Cultured Tobacco Cells
- Early events induced by aluminum stress in elongating cells of wheat root
- Aluminum Inhibits Growth and Stability of Cortical Microtubules in Wheat (Triticum aestivum) Roots
- Putative Ca^ Channels of Plasma Membrane Vesicles Are Not Involved in the Tolerance Mechanism of Aluminum in Aluminum Tolerant Wheat (Triticum aestivum L.) Cultivar
- Possible Involvement of Abscisic Acid in Increases in Activities of Two Vacuolar H^+ -Pumps in Barley Roots under Aluminum Stress
- In vivo Treatments That Modulate PP_i-Dependent H^+ Transport Activity of Tonoplast-Enriched Membrane Vesicles from Barley Roots
- Aluminum Stress Increases K^+ Efflux and Activities of ATP- and PP_i-Dependent H^+ Pumps of Tonoplast-Enriched Membrane Vesicles from Barley Roots : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
- EN-P24 Flow cytometry and cell-sorting method for isolating live bacteria with meta-cleavage activity on dihydroxy compounds of biphenyl(Section VI Environmental Biotechnology)
- Rapid and multiple in situ identification and analyses of physiological status of specific bacteria based on fluorescent in situ hybridization.
- Synergistic Inhibition of Growth by Aluminum and Iron of Tobacco (Nicotiana tabacum L.)Cells in Suspension Culture : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
- Quantitative Estimation of Aluminium Toxicity in Cultured Tobacco Cells : Correlation between Aluminium Uptake and Growth Inhibition : GROWTH AND DEVELTOPMENT : ENVIRONMENTAL AND STRESS RESPONSES
- Studies on the Mechanism of Aluminum Tolerance in Pea (Pisum sativum L.) Using Aluminum-Tolerant Cultivar 'Alaska' and Aluminum-Sensitive Cultivar 'Hyogo'(Plant Nutrition)
- Responses to Aluminium of Suspension-Cultured Tobacco Cells in a Simple Calcium Solution
- Cell Death Process Initiated by a Combination of Aluminium and Iron in Suspension-Cultured Tobacco Cells (Nicotiana tabacum): Apoptosis-Like Cell Death Mediated by Calcium and Proteinase
- Enhancement of Callose Production by a Combination of Aluminum and Iron in Suspension-Cultured Tobacco (Nicotiana tabacum) Cells
- Oxidative Damage to Membranes by a Combination of Aluminum and Iron in Suspension-Cultured Tobacco Cells
- Stimulation of H^+ Extrusion and Plasma Membrane H^+-ATPase Activity of Barley Roots by Ammonium-Treatment
- Effects of in Vivo Treatment with Abscisic Acid and/or Cytokinin on Activities of Vacuoler H^+ Pumps of Tonoplast-Enriched Membrane Vesicles Prepared from Barley Roots
- Characterization of the TaALMT1 Protein as an Al^-activated Anion Channel in Transformed Tobacco (Nicotiana Tabacum L.) Cells
- Cloning and Expression of an Ether-Bond-Cleaving Enzyme Involved in the Metabolism of Polyethylene Glycol(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- 3B10-1 The physiological role of glutathione-S-transferase in the downstream of peg operon in Sphingopyxis macrogoltabida strain 103
- Closing Plant Stomata Requires a Homolog of an Aluminum-Activated Malate Transporter
- 9-14 The resistant mechanism to iron deficiency in red clover. II.The characteristics and functions of root exudates
- Characterization of a Cryptic Plasmid, pSM103mini, from Polyethylene-Glycol Degrading Sphingopyxis macrogoltabida Strain 103
- Changes in the Structure of Chromatin in Pea Shoots upon a Shift in Growth Temperature from 2℃ to 25℃
- Heterogeneity of Dehydrogenases of Stenotrophomonas maltophilia Showing Dye-linked Activity with Polypropylene Glycols(Microbiology & Fermentation Technology)
- CLONING AND SEQUENCING OF THE cDNAsINDUCED BY ALUMINIUM TREATMENT IN TOBACCO CELLS
- Culturable bacteria in hydroponic cultures of moss Racomitrium japonicum and their potential as biofertilizers for moss production(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Proposed Oxidative Metabolic Pathway for Polypropylene Glycol in Sphingobium sp. Strain PW-1
- 2H11-3 Quantitative Analysis of an Anaerobic Benzene Degrader in Contaminated Underground Water Based on Fluorescence In Situ Hybridization
- Characterization of an Anion Transporter in the Plasma Membrane of Barley Roots
- Stimulation of Plasma Membrane H^+-Transport Activity in Barley Roots by Salt Stress
- Recent Developments in the Study of Microbial Aerobic Degradation of Polychlorinated Biphenyls
- The Biochemistry and Molecular Biology of Xenobiotic Polymer Degradation by Microorganisms
- In Vivo Treatment of Barley Roots with Vanadate Increases Vacuolar H^+-Translocating ATPase Activity of the Tonoplast-Enriched Membrane Vesicles and the Level of Endogenous ABA : MEMBRANES AND BIOENERGETICS
- The Impact of Aluminium on the Distribution of Cell Wall Glycoproteins of Pea Root Tip and Their Al-Binding Capacity
- Reconstitution of Plasma Membrane ATPase from Mature Cucumber Roots
- Changes of Some Properties of the Plasma Membrane-Enriched Fraction of Barley Roots Related to Aluminum Stress : Membrane-Associated ATPase, Aluminum and Calcium
- Assessment of change in biofilm architecture by nutrient concentration using a multichannel microdevice flow system(METHODS)
- Dominant Colonization and Inheritance of Methylobacterium sp. Strain OR01 on Perilla Plants
- Dominant Colonization and Inheritance of Methylobacterium sp. Strain OR01 on Perilla Plants