In vivo Treatments That Modulate PP_i-Dependent H^+ Transport Activity of Tonoplast-Enriched Membrane Vesicles from Barley Roots
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概要
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The PP_i-dependent H^+ transport activity of tonoplast-enriched membrane vesicles prepared from barley roots was greatly reduced when the plants were grown for 4 or 5 days with an additional 3 mM KCl in growth medium that contained only 0.1 mM CaCl_2 in water. To characterize the mechanism of this reduction in activity, we attempted to treat barley roots with K^+ ions, Cl^- ions (or acetate), and A23187 (with or without Ca^<2+> ions), which might be expected to cause alkalization, acidification and mobilization of Ca^<2+> ions in the cytoplasm, respectively. One-day treatment of barley roots with K^+ ions significantly decreased PP_i-dependent H^+ transport activity of prepared tonoplast-enriched membrane vesicles, while treatment with Cl^- ions or acetate significantly increased the activity. A similar increase in the activity also occurred by treatment with Ca^<2+> ions alone or in combination with A23187. Determination of the PP_i-hydrolyzing activity of membrane vesicles showed that changes in this activity by the various treatments were similar to those in the PP_i-dependent H^+ transport activity. The changes in ATP-dependent H^+ transport activity of membrane vesicles caused by these treatments were small. These results indicate that the in vivo treatments had significant effects on the H^+ transport activity of H^+_PP_i-ase, one of the two active vacuolar H^+_pumps (H^+_PP_iase and H^+_ATPase). In addition, these results suggest the possibility that changes in levels of cytoplasmic H^+ or Ca^<2+> ions may be involved in modulation of the H^+ transport activity of the vacuolar H^+_PP_iase during plant growth.
- 日本植物生理学会の論文
著者
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Yamamoto Yoko
Research Institute for Bioresources, Okayama University
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Yamamoto Yoko
Research Institute For Bioresources Okayama University
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MATSUMOTO Hideaki
Research Institute for Bioresources, Okayama University
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Kasai M
Department Of Botany Faculty Of Science Hirosaki University
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Kasai Minobu
Dept. Biofunc. Sci. Fac. Agr. Life Sci. Horisaki Univ.
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Matsumoto Hideaki
Research Institute For Bioresources Okayama University
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Sasaki Masao
Research Institute For Bioresources Okayama University
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Shibasaka M
Research Institute For Bioresources Okayama University
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KASAI Minobu
Research Institute for Bioresources, Okayama University
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Kasai Minobu
Research Institute For Bioresources Okayama University:(present Address)department Of Biology Facult
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