Repression of Asolectin-Dependent Activation of Partially Lipid Depleted ATPase Prepared from the Plasma Membrane-Enriched Fraction of Cucumber Roots due to Ca^<2+> Starvation
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概要
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The ATPase activity of the plasma membrane-enriched fraction was severely inhibited by withdrawal of Ca^<2+> from the medium for 5 days, although the root system appeared to be unaffected to visual inspection. Partially lipid-depleted ATPases with similar ratios of phospholipid to protein were prepared from the plasma membrane-enriched fraction of cucumber roots cultured with control medium and one lacking Ca^<2+>, and their properties were compared. SDS disc polyacrylamide gel electrophoresis showed that the polypeptide components were essentially similar between control and Ca^<2+>-starved roots. Partially lipid-depleted ATPase reassociated with asolectin, the lecithin from soybean, showed typical characteristics of plasma membrane type ATPase; pH optimum at 6.5, high specificity for ATP as substrate and strong inhibition by vanadate but not nitrate. The activity of reassociated ATPase obtained from the control roots was apparently higher than the activity obtained from Ca^<2+>-starved roots. The amount of asolectin required for maximum activation of the partially lipid-depleted ATPase prepared from con-trol roots was much lower than that prepared from Ca^<2+>-starved roots. Reassociation of partially lipid-depleted ATPase with asolectin produced higher ATPase activity than that with in-dividual phospholipids. The activation of partially lipid-depleted ATPase prepared from control roots with asolectin was not inhibited by addition of a sample prepared from Ca^<2+>-starved roots. Thus, a decrease in the functional association of ATPase with phospholipids might be one of the physiological injuries in root cell membranes of cucumber caused by Ca^<2+> starvation.
- 日本植物生理学会の論文
著者
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Matsumoto Hideaki
Research Institute For Bioresources Okayama University
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Chung Gap
Research Institute For Bioresources Okayama University:(present)department Of Horticulture College O
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