Localization of the NaCl-Sensitive Membrane Fraction in Cucumber Roots by Centrifugation on Sucrose Density Gradients
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概要
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Cucumber plants (Cucumis sativus L. cv. Shogoin) were treated with 200 mM NaCl for one day and the root microsomal membranes were fractionated by centrifugation on a continuous density gradient of 10% to 45% sucrose, and the activity of K^+-Mg^<2+>-ATPase was studied. The most significant difference in the ATPase activity between control and NaCl-stressed roots was detected in the membranes sedimented at sucrose concentrations of 40% to 45%. The ATPase activity associated with those fractions was vanadate-sensitive, and the membranes contained much lower levels of calcium and phospholipids after the treatment with NaCl. The putative plasma membrane fraction collected after centrifugation on a discontinuous gradient of 34% and 45% sucrose appeared to originate from the plasma membrane, as judged by sensitivity to inhibitors and pH optimum of the ATPase activity. NaCl-stress caused a significant reduction in K^+-Mg^<2+>-ATPase activity despite the similarity in polypeptide components detected by SDS-polyacrylamide gel electrophoresis on disc gels. The effects of NaCl-stress on the levels of calcium and phospholipids in membranes are discussed, with reference to the disintegration of the membranes.
- 日本植物生理学会の論文
著者
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Matsumoto Hideaki
Research Institute For Bioresources Okayama University
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Chung Gap
Department Of Horticulture College Of Agriculture Chonnam National University
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