Scavengers for Peroxynitrite : Inhibition of Tyrosine Nitration and Oxidation with Tryptamine Derivatives, α-Lipoic Acid and Synthetic Compounds

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The inhibitory effects of various endogenous and synthetic compounds on the nitration and oxidation of L-tyrosine by peroxynitrite were examined. Nitrating and oxidizing activities were monitored by the formation of 3-nitrotyrosine and dityrosine with a HPLC-UV-fluorescence detector system, respectively. Glutathione, seroninand synthetis sulfur- and selenium-containing compounds inhibited both the nitration and oxidation reaction of L-tyrosine effectively. However, 5-methosytryptamine, melatonin and α-lipoic acid only inhibited the nitration reaction, and enhanced the formation of an oxidation product. This is important evidence that there are different intermediates in the nitrating and oxidizing reactions of L-tyrosine by peroxynitrite. It was suggested that 5-methoxytryptamine, melatonin and α-lipoic acid reacted only with the nitrating intermediate of peroxynitrite and inhibited nitration of L-tyrosine. Actually, the DNA stand breakage, which is believed to be a typical reaction of hydroxyl radical-like species, caused by peroxynitrite was not effectively ionhibited by 5-methoxytryptamine. 5-Methoxytryptamine, melatonin and α-lipoic acid were viewed as useful reagents for investigating the mechanisms of damage by peroxynitrite in vitro.
公益社団法人日本薬学会の論文
2000-02-01