Evaluation of Distribution Patterns for Copper and Zinc in Metallothionein and Superoxide Dismutase in Chronic Liver Diseases and Hepatocellular Carcinoma Using High-Performance Liquid Chromatography(HPLC)(Analytical Biochemistry)

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概要

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• It has been reported that the copper (Cu) content of hepatocytes increases in chronic liver diseases and small hepatocellular carcinoma (HCC). In cells, Cu exists mainly as Cu-metallothionein (MT) or Cu, zinc (Zn)-superoxide dismutase (SOD). In this study, we investigated the biochemical state of Cu in the hepatocytes of patients with HCC using high-performance liquid chromatography (HPLC). The subjects of present study were 23 patients with HCC who underwent liver resection. The cancerous tissue and non-cancerous hepatic parenchyma with chronic disease were analyzed. In addition, as a normal control, hepatic tissue was collected at autopsy from 13 patients with no liver disease. Each sample was diluted with buffer, chilled, homogenized, and centrifuged. The supernatant was fractionated using HPLC. The metal contents of each fraction were measured using a desktop-type inductively coupled plasma (ICP) emission spectrochemical analyzer. HPLC analysis showed that MT existed mainly as Zn-MT in the normal hepatic tissue. The case of Cu, Zn-MT was significantly greater than Zn-MT in the non-cancerous, but diseased hepatic parenchyma than in the normal hepatic tissue (p<0.01). In comparison with non-cancerous hepatic parenchyma, the Cu-MT in the cancerous section was significantly greater than the Cu, Zn-MT (p<0.01). The Cu content for MT was significantly higher in small HCC (<40mm)(p<0.01), and the absence of Cu or Zn in the MT fraction was significantly more frequent in the large HCC (≧40mm)(p<0.01). The Cu and Zn content for SOD in the samples showed no significant difference. Increase in the Cu content in the cancerous hepatic tissue were, thought to be reflecting changes in the distribution of Cu in the MT fraction of hepatic tissues.

• 公益社団法人日本薬学会の論文
• 2005-07-01

著者

• 伊古田 暢夫

  放射線医学総合研究所

• SAKURAI Hiromu

  Department of Analytical and Bioinorganic Chemistry, Kyoto Pharmaceutical University

• Saisho H

  Department Of Medicine And Clinical Oncology Graduate School Of Medicine Chiba University

• IKOTA Nobuo

  School of Pharmacy, Shujitsu University

• 伊古田 暢夫

  放射線医学総合研究所重粒子医科学センター

• Ishigure Nobuhito

  Env. Toxic. Sci. Cent. Radiat. Safety Nat. Inst. Rad. Sci.(nirs)

• SAISHO Hiromitsu

  First Department of Internal Medicine, School of Medicine, Chiba University

• OZAWA Toshihiko

  Department of Health Pharmacy, Yokohama College of Pharmacy

• Ikota Nobuo

  National Institute Of Radiological Sciences

• Ikota Nobuo

  Research Center For Radiation Emergency Medicine National Institute Of Radiological Sciences

• Kubo Satoshi

  新潟薬科大学 薬理

• OZAWA Toshihiko

  National Institute of Radiological Sciences

• Yoshikawa M

  Environmental Radiation Protection Research Group National Institute Of Radiological Sciences

• YUKAWA Masae

  National Institute of Radiological Sciences

• Ishii Nobuyuki

  Env. Toxic. Sci. Cent. Radiat. Safety Nat. Inst. Rad. Sci.(nirs)

• Ikota Nobuo

  Redox Regulation Research Group National Institute Of Radiological Sciences

• KUBO Satoshi

  First Department of Medicine, School of Medicine, Chiba University

• FUKUDA Hiroyuki

  First Department of Medicine, School of Medicine, Chiba University

• EBARA Masaaki

  First Department of Medicine, School of Medicine, Chiba University

• NAKAGAWA Hidehiko

  National Institute of Radiological Sciences

• KATO Kazuki

  First Department of Medicine, School of Medicine, Chiba University

• SATOH Tsunenobu

  The Second Department of Pathology, Chiba University

• WATAYO Takaho

  Department of Surgery, Tokyo Metropolitan Ebara Hospital

• Watayo Takaho

  Department Of Surgery Tokyo Metropolitan Ebara Hospital

• Ebara M

  First Department Of Medicine School Of Medicine Chiba University

• Ebara Masaaki

  First Department Of Medicine Chiba University School Of Medicine

• Satoh Tsunenobu

  The Second Department Of Pathology Chiba University

• Ozawa Toshihiko

  Radiation Modifier Research Team Heavy-ion Radiobiology Research Group Research Center For Charged P

• Nakagawa Hidetoshi

  Faculty Of Pharmaceutical Sciences Kumamoto University

• Kato Kazuki

  Second Department Of Phthology School Of Medicine Chiba University

• Fukuda H

  Chiba Univ.

• Ozawa Toshihiko

  放射線医学総合研究所重粒子医科学センター 粒子線生物研究グループ

• Yukawa Masae

  放射線医学総合研究所

• Sakurai Hiromu

  Department Of Analytical And Bioinorganic Chemistry Kyoto Pharmaceutical University

• Nakagawa H

  Mukogawa Women's Univ. Nishinomiya Jpn

• Fukuda Hiroyuki

  Department Of Analytical And Bioinorganic Chemistry Kyoto Pharmaceutical University

• Nakagawa Hiroshi

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• Nakagawa Hidehiko

  Department Of Organic And Medicinal Chemistry Graduate School Of Pharmaceutical Sciences Nagoya City

• Ozawa Toshihiko

  National Institute Of Radiological Science

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