Quantitative Measurements of Oxidative Stress in Mouse Skin Induced by X-Ray Irradiation
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概要
- 論文の詳細を見る
To find efficient methods to evaluate oxidative stress in mouse skin caused by X-ray irradiation, several markers and methodologies were examined. Hairless mice were irradiated with 50Gy X-rays and skin homogenates or skin strips were prepared. Lipid peroxidation was measured using the skin homogenate as the level of thiobarbituric acid reactive substances. The level of lipid peroxidation increased with time after irradiation and was twice that of the control at 78h. ESR spectra of skin strips showed a clear signal for the ascorbyl radical, which increased with time after irradiation in a manner similar to that of lipid peroxidation. To measure levels of glutathione (GSH) and its oxidized forms (GSSG) simultaneously, two HPLC methods, sample derivatization with 1-fluoro-2, 4-dinitrobenzene and detection with a UV detector (method A) and no derivatization and detection with an electrochemical detector (method B), were compared and the latter was found to be better. No significant change was observed within 24h after irradiation in the levels of GSH and GSSG measured by method B. The GSH/GSSG ratio may be a less sensitive parameter for the evaluation of acute oxidative stress caused by X-ray irradiation in the skin. Monitoring the ascorbyl radical seems to be a good way to evaluate oxidative stress in skin in vivo.
- 公益社団法人日本薬学会の論文
- 2005-11-01
著者
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伊古田 暢夫
放射線医学総合研究所
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YAMAMOTO Haruhiko
Department of Applied Bioscience, Faculty of Science, Kanagawa University
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ANZAI Kazunori
Redox Regulation Research Group, Research Center for Radiation Safety, National Institute of Radiolo
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OZAWA Toshihiko
Redox Regulation Research Group, Research Center for Radiation Safety, National Institute of Radiolo
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IKOTA Nobuo
Redox Regulation Research Group, Research Center for Radiation Safety, National Institute of Radiolo
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URANO Shiro
Department of Applied Chemistry, Shibaura Institute of Technology
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IKOTA Nobuo
School of Pharmacy, Shujitsu University
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伊古田 暢夫
放射線医学総合研究所重粒子医科学センター
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Urano S
Division Of Biochemistry Shibaura Institute Of Technology
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Urano Shiro
青森大学
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Urano Shiro
芝浦工業大学 生化学
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Urano S
Department Of Applied Chemistry Shibaura Institute Of Technology
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Urano Shiro
Department Of Applied Chemistry Shibaura Institute Of Technology
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Ishigure Nobuhito
Env. Toxic. Sci. Cent. Radiat. Safety Nat. Inst. Rad. Sci.(nirs)
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OZAWA Toshihiko
Department of Health Pharmacy, Yokohama College of Pharmacy
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Ikota Nobuo
Research Center For Radiation Emergency Medicine National Institute Of Radiological Sciences
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Tanaka Ryoko
Redox Regulation Research Group National Institute Of Radiological Sciences:department Of Applied Bi
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Anzai Kazunori
Radiation Modifier Research Team Heavy-ion Radiobiology Research Group Research Center For Charged P
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Anzai Kazunori
Redox Regulation Research Group National Institute Of Radiological Sciences
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Ishii Nobuyuki
Env. Toxic. Sci. Cent. Radiat. Safety Nat. Inst. Rad. Sci.(nirs)
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Ikota Nobuo
Redox Regulation Research Group National Institute Of Radiological Sciences
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CHI Cuiping
Redox Regulation Research Group, National Institute of Radiological Sciences
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OKUDA Yohei
Redox Regulation Research Group, National Institute of Radiological Sciences
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Ozawa Toshihiko
Radiation Modifier Research Team Heavy-ion Radiobiology Research Group Research Center For Charged P
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Ozawa Toshihiko
Redox Regulation Research Group National Institute Of Radiological Sciences
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Ozawa Toshihiko
放射線医学総合研究所重粒子医科学センター 粒子線生物研究グループ
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Okuda Yohei
Redox Regulation Research Group National Institute Of Radiological Sciences:department Of Applied Ch
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Yamamoto Haruhiko
Department Of Applied Bioscience Faculty Of Science Kanagawa University
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Chi Cuiping
Research Center For Charged Particle Therapy National Institute Of Radiological Sciences
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