An Improved Diagnostic Method for Chronic Hepatic Disorder : Analyses of Metallothionein Isoforms and Trace Metals in the Liver of Patients with Hepatocellular Carcinoma as Determined by Capillary Zone Electrophoresis and Inductively Coupled Plasma-Mass S

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概要

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• It is desirable to diagnose hepatocellular carcinoma (HCC) in the early stages during its development since its treatment is usually difficult. We previously proposed a new diagnostic method that made use of the total metallothionein (MT), zinc (Zn), and copper (Cu) concentrations in the liver of the HCC patients. We recently found that MT-1 is involved in the metabolism or detoxification of toxic metals, such as cadmium; on the other hand, MT-2 is responsible for the homeostasis of essential metals such as copper, in experimental models such as Long Evans Cinnamon (LEC) rats. In order to device a better diagnostic method than the one we proposed previously, in this study, we newly propose an improved method that includes the discriminative determination data regarding the MT isomers, namely, MT-1 and MT-2, in the liver of patients with or without HCC as compared with the total MT level. The total MT and Zn concentrations in the HCC patients were confirmed to be significantly lower than those in patients without hepatic disorders (Ctrl). In contrast, Cu concentrations of the HCC patients were higher than those of the Ctrl patients. In addition, in the juxta-tumor portion with HCC, MT-1 concentrations were significantly higher than those of MT-2. In contrast, the MT-1 concentrations in the tumor portion were significantly lower than that in the juxta-tumor portion. In addition, MT-1/MT-2 ratio in the tumor portion was significantly lower than that of the juxta-tumor portion. By using parameters such as concentrations of Cu, Zn, total MT, and MT isomers, we performed the multivariate discriminative analysis (MDA). The results suggest that the concentrations of MT isomers change depending on the progress of the tumor, and information on MT isomers and trace elements is very useful in determining the stage of the chronic hepatic disorder.

• 公益社団法人日本薬学会の論文
• 2006-03-01

著者

• SAKURAI Hiromu

  Department of Analytical and Bioinorganic Chemistry, Kyoto Pharmaceutical University

• Saisho H

  Department Of Medicine And Clinical Oncology Graduate School Of Medicine Chiba University

• Minami Takeshi

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• NAGAMINE TAKEAKI

  First Department of Internal Medicine, Gunma University School of Medicine

• FUKUDA HIROYUKI

  Department of Surgery, Omiya Red Cross Hospital

• EBARA Masaaki

  First Department of Medicine, School of Medicine, Chiba University

• Ebara M

  First Department Of Medicine School Of Medicine Chiba University

• Ebara Masaaki

  First Department Of Medicine Chiba University School Of Medicine

• NAKAYAMA Akihiro

  Department of Physics, Meijo University

• Fukuda H

  Chiba Univ.

• Sakurai Hiromu

  Department Of Analytical And Bioinorganic Chemistry Kyoto Pharmaceutical University

• Nakayama A

  Department Of Analytical And Bioinorganic Chemistry Kyoto Pharmaceutical University

• Minami T

  Osaka Univ. Osaka Jpn

• Minami T

  Department Of Science And Technology Kinki University

• Minami Takeshi

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• NAKAMURA Shunsuke

  Department of Cellular and Molecular Medicine, Graduate School of Medicine, Chiba University

• Fukuda Hiroyuki

  Department Of Analytical And Bioinorganic Chemistry Kyoto Pharmaceutical University

• KAWATA Takumi

  Department of Analytical and Bioinorganic Chemistry, Kyoto Pharmaceutical University

• NAKAYAMA Akihiro

  Gifu Keizai University

• Nagamine Takeaki

  First Department Of Internal Medicine And Clinical Laboratory Center Gunma University School Of Medi

• Nakamura Shunsuke

  Department Of Analytical And Bioinorganic Chemistry Kyoto Pharmaceutical University

• Nakayama Akihiro

  Department Of Analytical And Bioinorganic Chemistry Kyoto Pharmaceutical University

• Kawata Takumi

  Department Of Analytical And Bioinorganic Chemistry Kyoto Pharmaceutical University

• MINAMI TAKESHI

  Department of Biochemistry, Faculty of Pharmaceutical Sciences, University of Tokushima

• NAKAYAMA Akihiro

  Department of Physics, Nagoya University

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