In Vivo Nitric Oxide Production and iNOS Expression in X-Ray Irradiated Mouse Skin(Miscellaneous)
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概要
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Inducible nitric oxide synthase (iNOS) and NO have been suggested to be involved in acute radiation response in tissues such as the liver, intestine, colon, and brain. However, direct measurement of NO and iNOS in ionizing radiation-induced skin inflammatory reactions is not reported yet. We show here for the first time, by in vivo experiments, that X-ray irradiated mouse skin generates NO with concomitant expression of iNOS at both the mRNA and protein levels. When irradiated at 50Gy, iNOS mRNA appeared at day 8 post-irradiation, whereas iNOS protein could be detected only at day 14. No iNOS protein was detectable however for the mice receiving 5 or 15 Gy irradiation, even at day 14. Skin inflammatory reactions were observed at day 8 post-irradiation as an increase in skin thickness, which increased further by day 14. Histological observations showed acute inflammatory responses. The parallel relationship between iNOS induction and the onset of skin inflammatory reactions suggests the involvement of iNOS and NO in the skin damage. Immunohistochemical staining showed the localization of iNOS at skin erosion areas, exudate and infiltrating cells. Taken together, these findings suggest that iNOS induction and NO production in X-irradiated skin are relatively early events in skin inflammatory reactions and are probably secondary rather than primary reactions of irradiation.
- 公益社団法人日本薬学会の論文
- 2006-02-01
著者
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ANZAI Kazunori
Redox Regulation Research Group, Research Center for Radiation Safety, National Institute of Radiolo
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OZAWA Toshihiko
Redox Regulation Research Group, Research Center for Radiation Safety, National Institute of Radiolo
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OZAWA Toshihiko
Department of Health Pharmacy, Yokohama College of Pharmacy
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Anzai Kazunori
Radiation Modifier Research Team Heavy-ion Radiobiology Research Group Research Center For Charged P
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Anzai Kazunori
Redox Regulation Research Group National Institute Of Radiological Sciences
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CHI Cuiping
Redox Regulation Research Group, National Institute of Radiological Sciences
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Ozawa Toshihiko
Radiation Modifier Research Team Heavy-ion Radiobiology Research Group Research Center For Charged P
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Ozawa Toshihiko
Redox Regulation Research Group National Institute Of Radiological Sciences
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Ozawa Toshihiko
放射線医学総合研究所重粒子医科学センター 粒子線生物研究グループ
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Chi Cuiping
Research Center For Charged Particle Therapy National Institute Of Radiological Sciences
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