ガラス化保存後の牛体外成熟卵子の高生存率
スポンサーリンク
概要
- 論文の詳細を見る
Improving pregnancy rates associated with the use of cryopreserved human oocytes would be an important advance in human assisted reproductive technology (ART). Vitrification allows glasslike solidification of a solution without ice crystal formation in the living cells. We have attempted to improve the survival rates of oocytes by a vitrification technique using bovine models. In vitro matured oocytes with or without cumulus cells were vitrified with either 15.0% (v/v) ethylene glycol (EG) + 15% (v/v) dimethylsulfoxide (DMSO) + 0.5 M sucrose or 15% (v/v) EG + 15% (v/v) 1,2-propanediol (PROH) + 0.5 M sucrose, using `Cryotop or `thin plastic sticker, respectively. The oocyte survival rates after vitrifying-warming, and the capacity for fertilization and embryonic development were examined in vitro. The rate of embryonic development to blastocyst was significantly higher (P<0.05) in the oocytes vitrified with 15% (v/v) EG + 15% (v/v) PROH + 0.5 M sucrose than in the oocytes vitrified with 15% (v/v) EG + 15% (v/v) DMSO + 0.5 M sucrose (7.4% ± 4.1 vs. 1.7% ± 3.0, respectively). Oocytes vitrified without cumulus cells had a higher survival rate after thawing and a superior embryonic developmental capacity compared with oocytes vitrified with cumulus cells. Prolonged pre-incubation time after thawing adversely affected the rates of embryonic cleavage and development. These results indicate that in vitro matured bovine oocytes can be vitrified successfully with the mixture of the cryoprotectants, EG + PROH, the absence of cumulus cells for vitrification does not affect oocyte survival rate after warming, and vitrified and warmed oocytes do not require pre-incubation before in vitro fertilization.
- 日本繁殖生物学会の論文
- 2004-12-01
著者
-
Kato Osamu
Kato Ladies Clinic
-
KUWAYAMA Masashige
KATO Ladies Clinic
-
NAGAI Takashi
National Institute of Livestock and Grassland Science
-
Nagai T
National Institute Of Livestock And Grassland Sciences
-
Tan J
Division Of Reproductive Biology Department Of Obstetrics And Gynecology Mcgill University
-
CHIAN Ri-Cheng
Division of Reproductive Biology, Department of Obstetrics and Gynecology, McGill University
-
TAN Leonard
Division of Reproductive Biology, Department of Obstetrics and Gynecology, McGill University
-
TAN Justin
Division of Reproductive Biology, Department of Obstetrics and Gynecology, McGill University
-
Tan Justin
Division Of Reproductive Biology Department Of Obstetrics And Gynecology Mcgill University
-
Tan Leonard
Division Of Reproductive Biology Department Of Obstetrics And Gynecology Mcgill University
-
Chian Ri-cheng
Division Of Reproductive Biology Department Of Obstetrics And Gynecology Mcgill University
-
Nagai Takashi
National Inst. Of Livestock And Grassland Sci.
-
桑山 正成
Kato Ladies Clinic
関連論文
- IS-46 What is the most cost-effective IVF protocol? A Comparison of natural, ultra-mini, and mini IVF protocols among patients with diminished ovarian reserve (DOR)(Group6 Reproduction1,International Session)
- Quantification of Microcystis in a Eutrophic Lake by Simple DNA Extraction and SYBR Green Real-time PCR
- ブタ生体回収由来ガラス化胚の生存性の改善
- Comparison of the Growth Performances of Offspring Produced by a Pair of Cloned Cattle and Their Nuclear Donor Animals
- Introduction of Various Vietnamese Indigenous Pig Breeds and Their Conservation by Using Assisted Reproductive Techniques
- Effect of Cryoprotectant Composition on In Vitro Viability of In Vitro Fertilized and Cloned Bovine Embryos Following Vitrification and In-Straw Dilution
- 成熟培養時間がウシ胚盤胞期胚の性比におよぼす影響
- 豚卵母細胞のDNA損傷と体外発育に及ぼす卵巣の保存時間と温度の影響
- ウシ卵巣の前胞状卵胞および胞状卵砲における卵胞と卵母細胞の成長に関する対比成長測定学的研究
- ウシ卵子における卵核胞崩壊に対するブチロラクトン-Iの影響とその後の卵子成熟、受精および発生能
- The Effects of Manipulation Medium, Culture System and Recipient Cytoplast on In Vitro Development of Intraspecies and Intergeneric Felid Embryos
- Vitrification of Fully Grown and Growing Porcine Oocytes Using Germinal Vesicle Transfer
- Effect of Donor Cell Types on Developmental Potential of Cattle (Bos taurus) and Swamp Buffalo (Bubalus bubalis) Cloned Embryos
- Effects of Season and Reproductive Phase on the Quality, Quantity and Developmental Competence of Oocytes Aspirated from Japanese Black Cows
- Birth of Cloned Calves Derived from Cultured Oviductal Epithelial Cells of a Dairy Cow
- Birth of Cloned Calves Derived from Cultured Oviductal Epithelial Cells of a Dairy Cow
- Intracytoplasmic Sperm Injection in the Pig : Where is the Problem?
- The Blastocyst Production Rate and Incidence of Chromosomal Abnormalities by Developmental Stage in In Vitro Produced Porcine Embryos
- Effects of Cysteine During In Vitro Maturation of Porcine Oocytes Under Low Oxygen Tension on Their Subsequent In Vitro Fertilization and Development
- An approach for conservation of pig resources by xenograft of gonadal tissue to mice
- Effect of Centrifugation Treatment before Vitrification on the Viability of Porcine Mature Oocytes and Zygotes Produced In Vitro
- Effect of Donor Cell Types on Developmental Potential of Cattle (Bos taurus) and Swamp Buffalo (Bubalus bubalis) Cloned Embryos
- Development In Vivo and In Vitro to Blastocysts of Porcine Oocytes Matured and Fertilized In Vitro
- A Comparison of Cryotop and Solid Surface Vitrification Methods for the Cryopreservation of In Vitro Matured Bovine Oocytes
- A Comparison of Cryotop and Solid Surface Vitrification Methods for the Cryopreservation of In Vitro Matured Bovine Oocytes
- 体外生産ブタ胚の体外培養における培地中のピルビン酸塩/乳酸塩のグルコースへの置換時期が胚発生におよぼす影響
- Interspecies Nuclear Transfer Embryos Reconstructed from Cat Somatic Cells and Bovine Ooplasm
- Health Status and Productive Performance of Somatic Cell Cloned Cattle and Their Offspring Produced in Japan
- Effects on N, N-Dimethylglycine on the Development of In Vitro Produced Bovine Embryos
- Effects of N, N-Dimethylglycine on the Development of In Vitro Produced Bovine Embryos
- Developmental Competence of Cat Oocytes from Ovaries Stored at Various Temperature for 24h
- Effect of Cycloheximide on In Vitro Development of Electrically Activated Feline Oocytes
- Effect of Replacement of Pyruvate/Lactate in Culture Medium with Glucose on Preimplantation Development of Porcine Embryos In Vitro
- Growth characteristics and growth modeling of Microcystis aeruginosa and Planktothrix agardhii under iron limitation
- Comparison of the Growth Performances of Offspring Produced by a Pair of Cloned Cattle and Their Nuclear Donor Animals
- ガラス化保存後の牛体外成熟卵子の高生存率
- ブタ生体回収由来ガラス化胚の生存性の改善
- Improvement of Porcine Oocytes with Low Developmental Ability after Fusion of Cytoplasmic Fragments Prepared by Serial Centrifugation
- Evaluation of Developmental Competence of In Vitro-produced Porcine Embryos Based on the Timing, Pattern and Evenness of the First Cleavage and Onset of the Second Cleavage
- Relationships of Survival Time, Productivity and Cause of Death with Telomere Lengths of Cows Produced by Somatic Cell NuclearTransfer
- Effects of Season and Reproductive Phase on the Quality, Quantity and Developmental Competence of Oocytes Aspirated from Japanese Black Cows
- The Effect of Ovary Storage and In Vitro Maturation on mRNA Levels in Bovine Oocytes; A Possible Impact of Maternal ATP1A1 on Blastocyst Development in Slaughterhouse-derived Oocytes
- Death losses due to stillbirth, neonatal death and diseases in cloned cattle derived from somatic cell nuclear transfer and their progeny : a result of nationwide survey in Japan
- Vitrification of Fully Grown and Growing Porcine Oocytes Using Germinal Vesicle Transfer
- The Effects of Manipulation Medium, Culture System and Recipient Cytoplast on In Vitro Development of Intraspecies and Intergeneric Felid Embryos
- Cloned Cows with Short Telomeres Deliver Healthy Offspring with Normal-length Telomeres
- Relationships of Survival Time, Productivity and Cause of Death with Telomere Lengths of Cows Produced by Somatic Cell Nuclear Transfer
- Improvement of Porcine Oocytes with Low Developmental Ability after Fusion of Cytoplasmic Fragments Prepared by Serial Centrifugation
- The Effect of Ovary Storage and In Vitro Maturation on mRNA Levels in Bovine Oocytes : A Possible Impact of Maternal ATP1A1 on Blastocyst Development in Slaughterhouse-derived Oocytes
- In vitro production of porcine embryos : current status, future perspectives and alternative applications
- Constant transmission of mitochondrial DNA in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm
- In-situ growth rate of Microcystis spp. and their growth-limiting factors : use of cellular RNA content
- Factors Affecting Cryopreservation of Porcine Oocytes
- Influence of Intergeneric/Interspecies Mitochondrial Injection : Parthenogenetic Development of Bovine Oocytes after Injection of Mitochondria Derived from Somatic Cells
- Survival of embryos and calves derived from somatic cell nuclear transfer in cattle : a nationwide survey in Japan