体外生産ブタ胚の体外培養における培地中のピルビン酸塩/乳酸塩のグルコースへの置換時期が胚発生におよぼす影響
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概要
- 論文の詳細を見る
The effect of glucose supplementation at different times in in vitro culture on the developmental competence of in vitro produced (IVP) porcine embryos was examined. In Experiment 1, when IVP embryos were cultured in modified NCSU-37 supplemented with pyruvate and lactate (IVC-pyr/lac) for 0 h, 24 h, 48 h, 72 h, 96 h, or 118 h and subsequently in modified NCSU-37 supplemented with glucose (IVC-glu) until Day 6 (Day 0=day of in vitro fertilization), the rates of blastocyst formation were significantly higher in embryos cultured in IVC-pyr/lac for 24 or 48 h (24.4% and 23.0%, respectively) than in embryos cultured in IVC-pyr/lac for the whole culture period (14.5%). However, there were no significant differences between embryos obtained after the energy source replacement and embryos cultured in IVC-glu for the whole culture period on the rates (15.2%-24.4%, and 16.8% respectively). Replacement of pyruvate/lactate with glucose at 58 h of culture in Experiment 2 significantly enhanced the rate (31.3%) compared to those after replacement at 48 h, 53 h and 63 h of culture (20.6%, 20.8%, and 21.1%, respectively). In conclusion, replacement of pyruvate/lactate with glucose as the energy substrate was optimal at 58 h of culture for the development of porcine embryos to the blastocyst stage.
- 日本繁殖生物学会の論文
著者
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NAGAI Takashi
National Institute of Livestock and Grassland Science
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OTOI Takeshige
Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi Unive
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KARJA Ni
Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi Unive
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ONISHI Akira
Developmental Biology Department, National Institute of Agrobiological Sciences
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MEDVEDEV Sergey
Developmental Biology Department, National Institute of Agrobiological Sciences
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FUCHIMOTO Dai-Ichiro
Developmental Biology Department, National Institute of Agrobiological Sciences
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IWAMOTO Masaki
Developmental Biology Department, National Institute of Agrobiological Sciences
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