Canine Lat1: Molecular Structure, Distribution and Its Expression in Cancer Samples
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概要
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A full-length cDNA sequence of canine L-type amino acid transporter 1 (Lat1) was determined from a canine brain. The sequence was 1828 bp long and was predicted to encode 485 amino acid polypeptides. The deduced amino acid sequence of canine Lat1 showed 93.2% and 91.1% similarities to those of humans and rats, respectively. Northern blot analysis detected Lat1 expression in the cerebellum at 4 kb, and Western blot analysis showed a single band at 40 kDa. RT-PCR analysis revealed a distinct expression of Lat1 in the pancreas and testis in addition to the cerebrum and cerebellum. Notably, Lat1 expression was observed in the tissues of thyroid cancer, melanoma and hemangiopericytoma. Although the cancer samples examined were not enough, Lat1 may serve as a useful biomarker of cancer cells in veterinary clinic.
著者
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Ochiai Hideharu
Research Institute Of Biosciences
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Morishita Taiki
Research Institute Of Biosciences Azabu University
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Onda Ken
Laboratories Of Veterinary Internal Medicine Iii
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Maruo Takuya
Veterinary Teaching Hospital
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ONDA Ken
Laboratory of Internal Medicine 3 Azabu University, School of Veterinary Medicine, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan
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SUGIYAMA Hiroki
Veterinary Teaching Hospital, Azabu University, School of Veterinary Medicine, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan
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MARUO Takuya
Veterinary Teaching Hospital, Azabu University, School of Veterinary Medicine, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan
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MORISHITA Taiki
Research Institute of Biosciences, Azabu University, School of Veterinary Medicine, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan
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SUGIYAMA Hiroki
Veterinary Teaching Hospital, Azabu University
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