Canine Amino Acid Transport System Xc−: cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells
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概要
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The cystine transport activity of a lens epithelial cell line originated from a canine mature cataract was investigated. The distinct cystine transport activity was observed, which was inhibited to 28% by extracellular 1mM glutamate. The cDNA sequences of canine cysteine/glutamate exchanger (xCT) and 4F2hc were determined. The predicted amino acid sequences were 527 and 533 amino acid polypeptides, respectively. The amino acid sequences of canine xCT and 4F2hc showed high similarities (>80%) to those of humans. The expression of xCT in lens epithelial cell line was confirmed by western blot analysis. RT-PCR analysis revealed high level expression only in the brain, and it was below the detectable level in other tissues.
- 公益社団法人 日本獣医学会の論文
著者
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Ochiai Hideharu
Institute Of Biosciences School Of Veterinary Medicine Azabu University
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Onda Ken
Laboratories Of Veterinary Internal Medicine Iii
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Kanemaki Nobuyuki
Veterinary Teaching Hospital
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Maruo Takuya
Veterinary Teaching Hospital
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OCHIAI Hideharu
Institute of Biosciences, School of Veterinary Medicine, Azabu University
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SATO Reiichiro
Laboratory of Gynecology and Obstetrics, School of Veterinary Medicine, Azabu University
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ICHIHARA Nobuteru
Laboratory of Anatomy 1, School of Veterinary Medicine, Azabu University
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KANEMAKI Nobuyuki
Veterinary Teaching Hospital, School of Veterinary Medicine, Azabu University
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