Structural and Biological Identity of Recombinant EDF (Erythroid Differentiation Factor) with Natural EDF (Biological Chemistry)
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概要
- 論文の詳細を見る
EDF (Drythroid Differentiation Factor), which exhibits extensive defferentiation inducing activity toward mouse Friend leukemia (MEL) cells, is produced by human THP-1 cells stimulated with TPA. The protein is a homo-dimer linked by disulfide bonds, the subunit of which has a molecular weght of 15.5kd. We isolated cDNA clones encoding the 15.5kd subunit of EDF and successed in the latter's expression in Chinese Hamster Ovary (CHO) cells. That is, the CHO cells secreted a protein prosessing differentiation inducing activity. This paper reports the identity of this protein, termed recombinant EDF (r-EDF), with the natural EDF from THP-1 cells as to molecular weight, isoelectric point, amino acid sequence of the N-terminal, amino acid composition peptide map and the specific biological activity toward MEL cells.
- 社団法人日本農芸化学会の論文
- 1988-09-23
著者
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SHIBAI Hiroshiro
Central Research Laboratories, Ajinomoto Co., Inc.
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Eto Y
Ajinomoto Inc. Kawasaki Jpn
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Eto Yuzuru
Central Research Laboratories Ajinomoto Co. Inc.
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Tsuji T
Ajinomoto Co. Inc. Kawasaki Jpn
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Tsuji Tomoko
Central Research Laboratories Ajinomoto Co. Inc.
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Murata Masahiro
Central Research Laboratories Of Ajinomoto Co. Inc.
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Shibai H
Central Research Laboratories Of Ajinomoto Co. Inc.
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Shibai Hiroshiro
Central Research Laboratories Ajinomoto Co. Inc.
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ONOMICHI Kazuya
Central Research Laboratories, Ajinomoto Co., Inc.,
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Onomichi Kazuya
Central Research Laboratories Ajinomoto Co. Inc.
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