Enzymatic Production of Ribavirin from Purine Nucleosides by Brevibacterium acetylicum ATCC 954(Microbilolgy & Fermentation Industry)

概要

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Ribavirin (1-β-D-ribofuranosyl-1, 2, 4-triazole-3-carboxamide ; Virazole^[○!R]), which is known as a potent antiviral agent, was synthesized directly from purine nucleosides and 1, 2, 4-triazole-3-carboxamide (TCA) by a newly selected bacterium, Brevibacterium acetylicum ATCC 954. Among the purine nucleosides tested, guanosine and xanthbosine were selected as the best substrates as to ribavirin production. The highest amount of ribavirin produced was 229mM from 300mM guanosine and 300mM TCA on 96hr reactio at 60℃. In this system involving ATCC 954, rebavirin was effectively even produced at a limited concentration of phosphate (about 10 mM). ATCC 954 could also produce ribavirin directly from inosine and TCA in a high yield, but could hardly produce it directly from adenosine and TCA. In contrast, a bacterium belonging to the genus, Bacillus, could produce ribarivin directly from adenosine and TCA in a high yield.
社団法人日本農芸化学会の論文
1988-07-23