Glutamate Metabolism in a Glutamate-producing Bacterium, Brevibacterium flavum

概要

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Brevibacterium flavum No. 2247 was found to grow with L-glutamate as the sole carbon and nitrogen source on an agar-plate medium when high concentrations of L-glutamate, FeSO4 and biotin were added to the medium. It grew on L-glutamate in liquid medium only when yeast extract or high concentrations of FeSO4 and glucose or organic acids of the tricarboxylic acid cycle were added to the medium. The growth on L-glutamate in liquid medium was also stimulated by high concentrations of L-glutamate, biotin and MgSO4, and inhibited by a high concentration of (NH4)2SO4. Aspartate aminotransferase (TA)- and α-ketoglutarate dehydrogenase (KD)-defective mutants did not grow on L-glutamate, and glutamate-utilizing revertants derived from these mutants recovered TA and KD activity, respectively, whereas glutamate dehydrogenase (GD)-defective mutants grew on L-glutamate. Washed cells of strain No. 2247 grown on glutamate decomposed the amino acid, whereas those grown on glucose did not. The degradation was observed only under aerobic conditions. The former cells showed higher KD, succinate dehydrogenase and fumarase activities than the latter cells. Of 75 mutants which did not grow on glutamate but grew on succinate, three strains lacked KD but showed the same glutamate productivity as the parent strain. Four other strains with normal KD levels showed higher glutamate productivity than the parent.
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