Glutamate Transport and Production in Brevibacterium flavum

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L-Glutamate was taken up and concentrated into washed cells of Brevibacterium flavum, a glutamate-producing bacterium. Potassium ions specifically promoted the uptake rate. The uptake activity in the presence of K+ was inhibited by carbonyl cyanide-m-chlorophenyl hydrazone but not by N, N-dicyclohexylcarbodiimide, NaN3, ouabain, valinomycin or gramicidin. The activity was specifically and competitively inhibited by L-aspartate among L-amino acids but not so strongly by other structural analogues including D-glutamate. L-Aspartate uptake was also competitively inhibited by L-glutamate. Michaelis constants for glutamate and aspartate were 19 and 25jum, respectively. The glutamate uptake activity of the cells increased whenNH4+ in the culture medium was reduced. Five out of six mutants which were unable to grow on glutamate and produced more glutamate than the parent showeda lower maximumrate of uptake than the parent.
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