Breeding of Phenylalanine-producing Brevidacterium flavum Strains by Removing Feedback Regulation of Both the Two Key Enzymes in Its Biosynthesis (Microbiology & Fermentation Industry)

概要

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The growth of a mFP-resistant Brevibacterium flavum mutant, No. 221-43, having PDT^R was synergistically and completely inhibited by mFP plus Tyr-Glu, but not by mFP plus tyrosine of pFP plus Tyr-Glu, whereas that of a mutant having DS^R was only partially inhibited by mFP plus Tyr-Glu. Tyr-Glu could replace tyrosine required for the growth of tyrosine auxotroph. The phenylalanine uptake was competitively inhibited by tyrosine and the tyrosine uptake by phenylalanin. The phenylalanine uptake was also inhibited by mFP, but not by Tyr-Glu. Mutants having both PDT^R and DS^R derived from strain No. 221-43 were dffectively selected by the resistance to mFP plus Tyr-Glu, and produced much amounts of phenylalanine, with small amounts of tyrosine, than the parent. By the same method, mutants having DS^R and PDT^R, which produced 23.4 g/l of phenylalanine at maximum, were obtained from a pFP-resistant tyrosine anxotroph having PDT^R which produced 18 g/l. Similar mutants were also obtained from a tryptophan-producing strain, but produced smaller amounts of tryptophan than the parent, whereas the total amounts of tryptophan and phenylalanine produced were increased.
社団法人日本農芸化学会の論文
1988-09-23