Production of L-Tryptophan by Azaserine-, 6-Diazo-5-oxo-L-norleucine- and Cinnamate-resistant Mutants of Bacillus subtilis K
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概要
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Azaserine (AzaSer) and 6-diazo-5-oxo-L-norleucine (DON), which are glutamine antagonists, strongly inhibited the activity of anthranilate synthetase of Bacillus subtilis K. Mutant strains AJ 11980 (AzaSerr) and AJ 11981 (AzaSerr, DONr) showed increased accumulation of L-Trp compared with the parent strain, AJ 11979 (5FTr, IMr). Furthermore, we obtained from AJ 11981 mutant, AJ 11982, resistant to cinnamate, which was shown to block the common pathway of aromatic amino acid biosynthesis. AJ 11982 accumulated 13.6 g/1 of L-Trp from 130 g/1 of glucose in a flask culture. We optimized the conditions for large-scale cultivation of this mutant and L-Trp accumulation of 21.5 g/1 was attained using ajar fermentor.
- 社団法人 日本農芸化学会の論文
著者
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Enei Hitoshi
Central Research Laboratories Of Ajinomoto Co. Inc.
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Takenouchi Tomoharu
Central Research Laboratories Of Ajinomoto Co. Inc.
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Morinaga Yasushi
Central Research Laboratories Ajinomoto Co. Inc.
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Kurahashi Osamu
Central Research Laboratories Ajinomoto Co. Inc.
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Akashi Kunihiko
Central Research Laboratories Of Ajinomoto Co. Inc.
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NODA-WATANABE Mayumi
Central Research Laboratories of Ajinomoto Co., Inc.
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TORIDE Yasuhiko
Central Research Laboratories of Ajinomoto Co., Inc.
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