Improved _L-Threonine Production by the Amplification of the Gene Encoding Homoserine Dehydrogenase in Brevibacterium lactofermentum(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
A 3.2kb chromosomal DNA fragment encoding homoserine dehydrogenase (HD) of Brevibacterium lactofermentum M-15, a L-threonine and L-lysine producing mutant, was cloned into the unique PstI site of vector plasmid pAJ1844. Strain M-15 was transformed with the obtained recombinant plasmid pAJ210. The transformant, AJ12021, showed 2-fold higher HD activity and accumulated a 1.4-fold higher amount of L-threonine (maximally 25.0g/liter) than in the case of the host strain, M-15, respectively. The correlation between the amino acid production and the regulation of enzyme activities was discussed.
- 社団法人日本農芸化学会の論文
- 1987-01-23
著者
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Ishida Masaaki
Central Research Laboratories of Ajinomoto Co., Inc.
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Nakamori Shigeru
Central Research Laboratories of Ajinomoto Co., Inc.
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SANO Konosuke
Central Research Laboratories, Ajinomoto Co., Inc.
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Sano K
Ajinomoto Co. Inc. Kawasaki Jpn
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Sano Konosuke
Central Research Laboratories Of Ajinomoto Co. Inc.
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Ishida Masaaki
Central Research Laboratories Of Ajinomoto Co. Inc.
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Sano Konosuke
Central Research Laboratories Ajinomoto Co. Inc.
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Matsui Kazuhiko
Central Research Laboratories Ajinomoto Co. Ltd.
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Nakamori Shigeru
Central Research Laboratories Ajinomoto Co. Inc.
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Takagi H
Nagoya Univ. Aichi Jpn
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Takagi H
Murata Manufacturing Co. Ltd. Kyoto Jpn
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Ito K
Tohoku Univ. Sendai Jpn
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MIWA Kiyoshi
Central Research Laboratories, Ajinomoto Co., Inc.
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TAKAGI Hiroshi
Central Research Laboratories of Ajinomoto Co., Inc.
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ITO Koichi
Central Research Laboratories of Ajinomoto Co., Inc.
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Miwa K
Ajinomoto Co. Inc. Kawasaki Jpn
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Miwa Kiyoshi
Central Research Laboratories Ajinomoto Co. Inc.
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Matsui Katsuhiko
Department Of Biological Chemistry School Of Pharmacy Hokuriku University
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Nakamori S
Ajinomoto Co. Inc. Kawasaki Jpn
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Takagi Hiroshi
Central Research Laboratories Ajinomoto Co. Inc.
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