Purification and Characterization of 0-Acetylserine Sulfhydrylase of Corynebacterium glutamicum
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概要
- 論文の詳細を見る
We highly purified O-acetylserine sulfhydrylase from the glutamate-producing bacterium Corynebacterium glutamicum. The molecular mass of the purified enzyme was 34,500 as determined by SDS-polyacrylamide gel electrophoresis, and 70,800 as determined by gel filtration chromatography. It had an apparent Km of 7.0 mM for O-acetylserine and a Vmax of 435 μmol min−1 (mg·protein)−1. This is the first report of the cysteine biosynthetic enzyme of C. glutamicum in purified form.
- 社団法人 日本農芸化学会の論文
- 2004-07-23
著者
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和田 政裕
Faculty Of Pharmaceutical Sciences Josai University
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TAKAGI Hiroshi
Department of Surgery II, University School of Medicine
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HIRAI TAKANORI
Komaki Municipal Hospital
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WADA Masaru
Department of Bioscience, Fukui Prefectural University
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NAKAMORI Shigeru
Department of Bioscience, Fukui Prefectural University
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Wada M
Osaka Univ. Osaka
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Wada Masaru
Department Of Biology College Of Liberal Arts And Sciences Tokyo Medical And Dental University
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Wada M
Tokyo Medical And Dental Univ. Chiba Jpn
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Takagi H
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University:(present Address)
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Takagi Hiroshi
Second Department Of Surgery Nagoya University
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Nakamori Shigeru
Dept. Biosci. Fukui Pref. Univ.
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Nakamori Shigeru
Department Of Bioscience Faculty Of Bioscience Fukui Prefectural University
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Wada Masaru
Department Of Bioscience Fukui Prefectural University
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Takagi H
Department Of Bioscience Fukui Prefectural University
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Takagi Hiroshi
Department Of Medicine And Molecular Science Gunma University Graduate School Of Medicine
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AWANO Naoki
Department of Bioscience, Fukui Prefectural University
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YAMAZAWA Hiroyuki
Department of Bioscience, Fukui Prefectural University
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Nakamori S
Department Of Bioscience Fukui Prefectural University
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Takagi H
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.
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Wada M
Division Of Applied Bioscience Research Faculty Of Agriculture Hokkaido University
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Takagi Hiroshi
Department Of Bioscience Fukui Prefectural University
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Wada Masaru
Department Of Biology College Of Liberal Arts And Sciences Tokyo Medical And Dental Univ.
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Wada Masaru
Department Of Biology And Chemistry College Of Liberal Arts And Sciences Tokyo Medical Dental Univer
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Yamazawa Hiroyuki
Department Of Bioscience Fukui Prefectural University
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Awano Naoki
Department Of Bioscience Fukui Prefectural University
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Takagi Hiroshi
Department Of Bioscience Faculty Of Bioscience Fukui Prefectural University
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