Molecular Cloning of the Gene for Microbial Transglutaminase from Streptoverticillium and Its Expression in Streptomyces lividans
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概要
- 論文の詳細を見る
The microbial transglutaminase (TGase)-producing strain S-8112 [Agric. Biol. Chem., 53, 2613-2617(1989)] was identified as a variant of Streptoverticillium mobaraense. We amplified a partial gene fragment by polymerase chain reaction (PCR) using oligonucleotides synthesized from the amino acid sequence of TGase, and cloned the gene for TGase using the PCR amplified fragment as a probe. The gene encoded a precursor of TGase consisting of 406 amino acid residues, which comprised the prepro region of 75 amino acid residues and the mature region of 331 amino acid residues. We expressed the TGase gene in Streptomyces lividans under a tyrosinase promoter, and found an active and mature recombinant enzyme, indicating the processing of the gene product.
- 社団法人日本農芸化学会の論文
- 1994-01-23
著者
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MOTOKI Masao
Food Research & Development Laboratories, Ajinomoto Co., Inc.
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Ando K
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Takagi H
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University:(present Address)
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MATSUURA Akira
Central Research Laboratories, Amano Pharmaceutical Co., Ltd.
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Matsuura A
Department Of Gastroenterology Aichi Cancer Center Hospital
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Motoki M
Ajinomoto Co. Inc. Kawasaki Jpn
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Takeuchi Kazuyuki
Research And Development Division Amano Prarmaceutical Co. Ltd.
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Takeuchi Kazuyuki
Tsukuba Research Laboratories Amano Pharmaceutical Co. Ltd.
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Takagi H
Fukui Prefectural Univ. Fukui Jpn
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Ando Keiichi
Tsukuba Research Laboratories of Amano Pharmaceutical Co., Ltd.
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Koikeda Satoshi
Tsukuba Research Laboratories of Amano Pharmaceutical Co., Ltd.
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Takagi Hiroshi
Food Research & Development Laboratories of Ajinomoto Co., Inc.
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Washizu Kinya
Tsukuba Research Laboratories, Amano Pharmaceutical Co., Ltd.
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Hirose Susumu
Central Research Laboratories, Amano Pharmaceutical Co., Ltd.
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Washizu Kinya
Tsukuba Research Laboratories Amano Pharmaceutical Co. Ltd.
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Koikeda Satoshi
Tsukuba Research Laboratories Amano Pharmaceutical Co. Ltd.
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Ando K
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd
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Miura A
Third Department Of Internal Medicine Akita University School Of Medicine
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Matsuura Akira
Department Of Gastroenterology Aichi Cancer Center Hospital
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Hirose S
Hokkaido Univ. Sapporo Jpn
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Takagi H
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.
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Matsuura Akira
Central Research Laboratories Amano Pharmaceutical Co. Ltd.
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