Purification and Characterization of a Novel Glucoamylase from Acremonium sp. YT-78
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概要
- 論文の詳細を見る
A novel glucoamylase (EC 3.2.1.3,1,4-α-D-glucan glucohydrolase) was purified to homogeneity from the culture filtrate of Acremonium sp. YT-78 isolated from soil. The enzyme had a molecular mass of 74 kDa by SDS-PAGE and 148±2 kDa by gel filtration, and its isoelectric point was 5.10. The optimum temperature and pH were around 50℃ and 5.0,respectively. The enzyme was stable in a pH range from 5.0 to 9.0 and at below 40℃. The enzyme hydrolyzed α-D-glucans in an exo-manner and the glucose residue released by the action of the enzyme was β-configuration. THe enzyme had stronger pullulan-hydrolyzing activity than any glucoamylase so far reported. Panose, a saccharide containing an α-1,6-glucosidic linkage, was hydrolyzed by the enzyme, but it showed no activity towards isomaltose or dextran.
- 社団法人日本生物工学会の論文
- 1996-04-25
著者
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Matsuura A
Department Of Gastroenterology Aichi Cancer Center Hospital
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Matsuura A
Amano Pharmaceutical Co. Ltd. Aichi Jpn
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MASE TAMIO
Research and Development Division, Amano Pharmaceutical Co. Ltd.,
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MATSUMIYA YUKO
Research and Development Division, Amano Pharmaceutical Co. Ltd.,
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MORI SHIGEHARU
Research and Development Division, Amano Pharmaceutical Co. Ltd.,
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MATSUURA AKIRA
Research and Development Division, Amano Pharmaceutical Co. Ltd.,
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Mase T
Process Research Process R&d Laboratories For Technology Development Banyu Pharmaceutical Co. Lt
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Mase Tamio
Research And Development Division Amano Pharmaceutical Co. Ltd.
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Mori S
Sagami Chemical Res. Center Kanagawa Jpn
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Matsumiya Yuko
Research And Development Division Amano Pharmaceutical Co. Ltd.
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Miura A
Third Department Of Internal Medicine Akita University School Of Medicine
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Matsuura Akira
Department Of Gastroenterology Aichi Cancer Center Hospital
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Mase T
Amano Pharmaceutical Co. Ltd. Aichi Jpn
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Matsumiya Y
Research And Development Division Amano Pharmaceutical Co. Ltd.
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