Transfructosylation of Thiol Group by β-Fructofuranosidases
スポンサーリンク
概要
- 論文の詳細を見る
β-Fructofuranosidase fructosylated not only the hydroxyl group but also the thiol group of 2-mercaptoethanol in a transfer reaction using sucrose as a donor substrate. The enzymes form Candida utilis and Sac-charomyces cerevisiae (baker's yeast) were effective catalysts for the thio-fructofuranosylation. The thio-fructosylation product was isolated by activated carbon chromatography and its structure was confirmed by Fab-mass spectrometry and NMR spectroscopy. The thio-fructofuranoside was synthesized effectively at around 3.0M for the acceptor concentration. The product increased with the sucrose concentration at least up to 1.9M. O-Fructofuranoside was simultaneously synthesized at an early stage of the reaction, although it was hydrolyzed on further incubation. On the contrary, the thio-furctofuranoside accumulated efficiently after syntheis, indicating it was very stable against the hydrolytic action of the β-fructofuranosidase.
- 社団法人日本農芸化学会の論文
- 2000-07-23
著者
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Murakami Hiromi
Osaka Municipal Technical Res. Inst.
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Shizuma Motohiro
Osaka Municipal Technical Research Institute
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KITAHATA Sumio
Osaka Municipal Technical Research Institute
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KISO TARO
Osaka Municipal Technical Research Institute
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Murakami H
Laboratory Of Cellular Regulation Technology Graduate School Of Genetic Resources Technology Kyushu
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NAKANO HIROFUMI
Osaka Municipal Technical Research Institute, and Yashiro Co, Ltd.,
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Shizuma Motohiro
Technochemistry Osaka Municipal Technical Research Institute
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Kitahata S
Department Of Bioscience And Biotecnology Shinshu University
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ARAUJO Teomar
Federal University of Pernambuco, Keizo Asami Laboratory of Imunopatology Cidad Universitaria
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SHIZUMA Motohiro
Department of Biochemistry, Osaka Municipal Technical Research Institute
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Kiso Taro
Department Of Biochemistry Osaka Municipal Technical Research Institute
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Araujo Teomar
Federal University Of Pernambuco Keizo Asami Laboratory Of Imunopatology Cidad Universitaria
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Murakami H
Osaka Municipal Technical Res. Inst.
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Kobayashi S
Fukui Univ. Technol. Fukui Jpn
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Shizuma Motohiro
Department Of Biochemistry Osaka Municipal Technical Research Institute
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Nakano H
Department Of Biochemistry Osaka Municipal Technical Research Institute
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Nakano H
Kyoto Prefectural Univ. Kyoto Jpn
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Nakano Hirofumi
Osaka Municipal Technical Research Institute
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Nakano Hirofumi
Department Of Chemistry Faculty Of Science Osaka University
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