Formation of Trehalose and Its 2-Deoxy Analogs through Condensation by a Trehalase from Lobosphaera sp.
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概要
- 論文の詳細を見る
A purified trehalase from Lobosphaera sp. catalyzed the condensation not only of D-glucose but also of 2-deoxy-D-glucose, yielding α, α'-trehalose and 2, 2'-dideoxy-α, α'-trehalose, respectively. Simultaneous incubation of D-glucose and 2-deoxy-D-glucose resulted in the formation of 2-deoxy-α, α'-trehalose in addition to the above disaccharides. The reaction under various conditions showed that the enzyme synthesized dideoxy-trehalose (yield, 16%) more than trehalose (5%). Monodeoxy-trehalose was produced most effectively in the reaction of D-glucose and 2-deoxy-D-glucose in a weight ratio of 2 : 5, and at this ratio, 6%, of the total weight of two substrates was converted to the product.
- 社団法人日本農芸化学会の論文
- 1994-08-23
著者
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MORIWAKI Masamitsu
San-Ei-Gen F.F.I., Inc.
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KITAHATA Sumio
Osaka Municipal Technical Research Institute
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NAKANO HIROFUMI
Osaka Municipal Technical Research Institute, and Yashiro Co, Ltd.,
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Kitahata S
Department Of Bioscience And Biotecnology Shinshu University
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WASHINO Tutomu
Research and Development Section, San-Ei Gen F. F. I., Inc.
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Washino Tutomu
Research And Development Section San-ei Gen F. F. I. Inc.
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Washino T
San‐ei Gen F. F. I. Inc. Osaka Jpn
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Washino Tsutomu
San-ei Gen F. F. I. Inc.
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Nakano Hirofumi
Osaka Municipal Technical Research Institute
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Moriwaki Masamitsu
Department Of Biotechnology The University Of Tokyo
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Nakano Hirofumi
Department Of Chemistry Faculty Of Science Osaka University
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Moriwaki Masamitsu
San-ei-gen F.f.i. Inc.
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WASHINO Tsutomu
SAN-EI Chemical Industries Ltd.
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