Enrichment of Ethyl Docosahexaenoate by Selective Alcoholysis with Immobilized Rhizopus delemar Lipase

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概要

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• We attempted to purify ethyl docosahexaenoate (E-DHA) by the alcoholysis of fatty acid ethyl esters with lipase. Fatty acid ethyl esters originating from tuna oil (E-DHA content, 23 mol%; E-tuna-23) wre used as starting materials, and Rhizopus delemar lipase immobilized on a ceramic carrier was used as a catalyst which acted only very weakly on E-DHA. Because the immobilized lipase did not exhibit the alcoholysis activiyy, it was activated by shaking at 30℃ for 24 h in the E-tuna-23/lauryl alcohol mixture to which water(2%) was added. The alcoholysis activity of the lipase increased markedly as a result of this pretreatment, but the hydrolysis activity also increased. The hydrolysis activity was completely reprssed by repeating the reaction after transferring the immobilized enzyme into a fresh E-tuna-23/lauryl alcohol mixture without adding additional water. Several factors affecting the alcoholysis of E-tuna-23 were investigated to determine the optimum reaction conditins. When alcoholysis was conducted at 30℃ with shaking in a reaction mixture containing E-tuna 23/lauryl alcohol(1 : 3,mol/mol) and the activated lipase (4% of the mixture volume), E-DHA was efficiently enriched in the ethyl ester fraction. By alcoholyzing E-tuna-23 with lauryl alcohol for 50 h under these reaction conditions, the E-DHA content was increased from 23 mol% to 52 mol% in a 90% yield. In addition, when the fatty acid ethyl esters, of which the E-DHA contents were 45 mol% and 60 mol%, were alcoholyzed for 50 h, the contents of E-DHA were increased to 72 mol% and 83 mol%, respectively. In these reacitons, the recovery of E-DHA in the ethyl ester fraciton was greater than 90%. We termed this new reaction system seletive alcoholysis because advantage of the fatty acid specificity of the lipase was taken in this reaciton system. To investigate the stability of the immobilized lipase, continual batch reactions were carried out by replacing the reaction mixture with a fresh E-tuna-23/lauryl alcohol mixture every 24h. The decrease in the extent of alcoholysis was only 15% even after the 47th batch reaction.

• 社団法人日本生物工学会の論文
• 1997-08-25

著者

• Tominaga Y

  Dep. Of Applied Biochemistry And Food Sci. Saga Univ.

• Yanagita Teruyoshi

  Lab. Of Nutrition Biochemistry Dep. Of Applied Biochemistry And Food Sci. Saga Univ.

• Shimada Y

  Department Of Biochemistry Osaka Municipal Technical Research Institute

• Shimada Y

  Department Of Physics Chuo University:tecmo

• Shimada Yuji

  Osaka Municipal Technical Research Institute

• NAGAO Toshihiro

  Osaka Municipal Technical Research Institute

• SUGIHARA Akio

  Faculty of Engineering, Tokushima Bunri University

• SUGIHARA Akio

  Osaka Municipal Technical Research Institute

• TOMINAGA Yoshio

  Osaka Municipal Technical Research Institute

• YODONO SHIZUKA

  Department of Agricultural Chemistry, School of Agriculture, Kinki University

• MARUYAMA KAZUAKI

  Maruha Corp.

• NAKANO HIROFUIMI

  Osaka Municipal Technical Research Institute

• KOMEMUSHI SADAO

  Department of Agricultural Chemistry, School of Agriculture, Kinki University

• Yanagita Teruyoshi

  Dep. Of Applied Biochemistry And Food Sci. Saga Univ.

• Tominaga Yoshio

  The Organ Transplant Center Nagoya Second Red Cross Hospital

• Sugihara A

  Faculty Of Engineering Tokushima Bunri University

• Yodono Shizuka

  Department Of Agricultural Chemistry School Of Agriculture Kinki University

• Nagao Toshihiro

  Biomaterials And Commodity Chemicals Res. Div. Osaka Municipal Technical Res. Inst.

• Nagao Toshihiro

  Department Of Biochemistry Osaka Municipal Technical Research Institute

• Komemushi Sadao

  Department Of Agricultural Chemistry Faculty Of Agriculture Kinki University

• Komemushi Sadao

  Department Of Agricultural Chemistry School Of Agriculture Kinki University

• Maruyama Kazuaki

  R&d Div. Maruha Corporation

• Nakano Hirofumi

  Department Of Chemistry Faculty Of Science Osaka University

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