Effect of Active Oxygen Species on the Productivity of Torularhodin by Rhodotorula glutinis No.21

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概要

• 論文の詳細を見る

• The effect of active oxygen species on the productivity of torularhodin, an effective antioxidant, by soil yeast, Rhodotorula glutinis no. 21, was examined. Methylene blue, methyviologen and AAPH [2.2´-azobis(2-amidinopropan) dihydrochloride] were used as generators of singlet oxygen, superoxide anion radicals and peroxy radicals, respectively. All of them indicated effectiveness at a dose of 1.0×10^<-10> to 3.0×10^<-6>M. Addition of these generators to the culture medium had almost no influence on the biosynthesis of β-carotene, whereas it had marked enhancement on those of torulene and torularhodin. Production of uric acid by xanthineoxidase remained unchanged at a torularhodin concentration of up to 100 μM. This result suggests that torularhodin does not directly affect the productivity of superoxide anions. It has been proved that torularhodin has a more potent effect on the scavenging of peroxyl radicals and inhibits substrate degradation by singlet oxygen more effectively than Jβ-carotene does. Continuous addition of methylene blue enhanced the torularhodin accumulation more markedly than single addition.

• 2002-03-25

著者

• MIKI WATARU

  Suntory Co., Ltd

• KOMEMUSHI SADAO

  School of Agriculture, Kinki University

• KOMEMUSHI SADAO

  Department of Agricultural Chemistry, School of Agriculture, Kinki University

• Miki Wataru

  Fuji Chemical Ind. Co. Ltd.

• Miki W

  Marine Biotechnol. Inst. (mbi) Shizuoka Jpn

• SAKAKI HIDEYUKI

  Department of Agricultural Chemistry, Faculty of Agriculture, Kinki University

• MIKI WATARU

  Research Institute for New Product Development, Suntory Limited

• Sakaki Hideyuki

  Kobe Creative Center Senju Pharmaceutical Co. Ltd

• Komemushi S

  School Of Agriculture Kinki University

• Komemushi Sadao

  Department Of Agricultural Chemistry Faculty Of Agriculture Kinki University

• NOCHIDE HIDESATO

  Department of Agricultural Chemistry, Faculty of Agriculture, Kinki University

• Nochide Hidesato

  Department Of Agricultural Chemistry Faculty Of Agriculture Kinki University

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